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pcdna3mciap1 mut

Plasmid

#11476

Depositor

Jon Ashwell

Article

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Insert

mciap1mut (Birc2 Mouse)

Expression

Mammalian

Mutation

582 to alanine

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-N-Myc_535-1102 USP7

Plasmid

#131246

Purpose

Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 535-1102. This truncat…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDN-G1TGt

Plasmid

#44508

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
tetR::yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-T1TCbt

Plasmid

#44514

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-PH-BTKx2

Plasmid

#183654

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Frog, Human)

Tags

X. laevis map2k1.L(32-44)-mCherry

Expression

Mammalian

Mutation

Amino acids 2-174 and 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

Plasmid

#111391

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
ChETA-mRuby2

Use

AAV

Tags

Myc and mRuby2

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

Plasmid

#104566

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-p65

Tags

3x HA and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hLaminB1

Plasmid

#103799

Purpose

BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hLaminB1 (CIB1, LMNB1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer:cAPX

Plasmid

#84736

Purpose

Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

APX1 (APX1 Mustard Weed)
HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-hPXR-LBD

Plasmid

#67769

Purpose

Expression GST-hPXR-LBD in bacteria

Depositor

Sridhar Mani

Article

Li et al J Biol Chem. 2013 May 10;288(19):13655-6

Insert

human pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 107 to 434 of human PXR

Promoter

Tac

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pETM41-EcLonA

Plasmid

#38330

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

Lon protease fragment (lon Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

Polyphosphate binding fragment of Lon protease. …

Promoter

T7

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-3XKD/Y1699C

Plasmid

#25367

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
ATF4 7: 5’3’ATF4.GFP

Plasmid

#21854

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Vector derived viral termination and poly-adenyla…

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98H-pRc/CMV

Plasmid

#22044

Depositor

William Kaelin

Article

Ohh et al Mol Cell. 1998 Jun . 1(7):959-68.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant, converting aa 98 from Tyrosine to H…

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pFastBacMam4_N_Flag_His8_eGFP_TEV

Plasmid

#178808

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Tags

Flag, His8, eGFP, TEV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.tTA.WPRE

Plasmid

#38057

Depositor

James Uney

Article

Lee et al J Gene Med. 2005 May;7(5):576-83.

Insert

tTA

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pmNET

Plasmid

#35627

Depositor

Howard Gu

Article

Han et al BMC Pharmacol. 2006 Mar 3;6:6.

Insert

NET (Slc6a2 Mouse)

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3 tTA-3

Plasmid

#27105

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

tetracycline transactivator

Use

Gateway Cloning

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pATP416-ptetO7-pphlO6-crtYBI

Plasmid

#165976

Purpose

Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Inserts

Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

C1281A, G1698A and C66A, C1359T

Promoter

Synthetic promoter (pphlO6), Synthetic promoter (…

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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wg186Fluc

Plasmid

#177436

Purpose

In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.

Depositor

Wendy Gilbert

Article

Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1

Insert

Firefly luciferase

Use

Luciferase; For in vitro transcription

Tags

None

Promoter

T7

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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