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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,939 results for: REP

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Showing: 10341 - 10360 of 22939 results

  5. pExp-sfGFP

    Plasmid
    #112573
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and sfGFP
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112573-plasmid-map-sequence-id-217227

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  6. pJAT30

    Plasmid
    #204294
    Purpose
    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Promoter
    MHC
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204294-plasmid-map-sequence-id-405490

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  7. LSB-hsa-miR-21-5p

    Plasmid
    #103345
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-21-5p target (MIR21 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103345-plasmid-map-sequence-id-195739

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  13. pExp-Trx

    Plasmid
    #112577
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TrxA and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112577-plasmid-map-sequence-id-217212

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  18. pRR-AR

    Plasmid
    #73045
    Purpose
    Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)
    Depositor
    David McMillen
    Article
    Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-
    Insert
    Androgen receptor (AR Human)
    Expression
    Yeast
    Mutation
    please see depositor comments below
    Promoter
    glyceraldehyde phosphate dehydrogenase (GPD)
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    73045-plasmid-map-sequence-id-134099

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Showing: 10341 - 10360 of 22939 results