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pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

Plasmid

#229810

Purpose

A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-AmCyan-stop-lox

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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USUV strain Vienna 2001 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228658

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Usutu virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

Usutu virus portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228653

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

MVEV portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

Plasmid

#212675

Purpose

Express tagged hRb phosphosite mutant

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Mutation

15 CDK phospho-sites are mutated to alanines, and…

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pWR76

Plasmid

#202776

Purpose

episomal fluorescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

Plasmid

#218973

Purpose

Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8

Expression

Mammalian

Mutation

mutant SRSF1 binding motif (AGTCTCCT)

Promoter

RSV LTR

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-PytR(PyOtR)-mCherry

Plasmid

#204870

Purpose

Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

M. mazei pyrrolysyl tRNA mutant "PyOtR"

Use

AAV

Expression

Mammalian

Mutation

U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

Promoter

U6

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13972 - pAAV-GfaABC1D-SYFP2-P2A-3XFLAG-10aa-H2B*-WPRE3-BGHpA (Alias: CN3972)

Plasmid

#208159

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196079

Purpose

Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXL2-coemiRFP670-PGK-Puro

Plasmid

#186168

Purpose

Knock-in gene targeting in marmoset cells at FOXL2 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

emiRFP670

Use

Marmoset targeting

Mutation

human codon-optimized

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-AtAFB2-mTagBFP2

Plasmid

#179889

Purpose

Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

AtAFB2 (AFB2 Synthetic, Mustard Weed)

Use

Lentiviral

Tags

mTagBFP2

Expression

Bacterial and Mammalian

Promoter

EF1

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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DOP1B_pcDNA6.2/EmGFP-Bsd

Plasmid

#176979

Purpose

Mammalian expression vector encoding DOP1B and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DOP1B (DOPEY2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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GET1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176985

Purpose

Mammalian expression vector encoding GET1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GET1 (GET1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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NDUFV3_pcDNA6.2/EmGFP-Bsd

Plasmid

#176946

Purpose

Mammalian expression vector encoding NDUFV3 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

NDUFV3 (NDUFV3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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HSF2BP_pcDNA6.2/EmGFP-Bsd

Plasmid

#176947

Purpose

Mammalian expression vector encoding HSF2BP and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HSF2BP (HSF2BP Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PE VQR

Plasmid

#162797

Purpose

All-in-one prime editor piggyBac transposon, VQR variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

CAG

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 SEC66-VAC8-RFP

Plasmid

#166843

Purpose

Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, SEC66 Budding Yeast)

Tags

RFP and SEC66 transmembrane domain

Expression

Yeast

Mutation

SEC66 transmembrane domain

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

Plasmid

#89994

Purpose

Donor template for generation of NGN3-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)

Use

CRISPR

Promoter

none

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

Plasmid

#111923

Purpose

Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_19μ

Tags

CoxVIIIx2

Expression

Mammalian

Mutation

E67D, F92W, E104D, D133E at CaM

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTI EF1alpha FLAG/HA-LCOR

Plasmid

#114444

Purpose

Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human LCOR coding sequence

Depositor

Adrian Bracken

Article

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

LCOR (LCOR Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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