We narrowed to 3,085 results for: multiplex

1. Lenti-sgSmad4/Cre

   Plasmid

   #89651

   Purpose

   Expresses a Smad4-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   gRNA targeting Smad4 (Smad4 Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PRLSS-SH2-KDEL

   Plasmid

   #209716

   Purpose

   Mammalian expression of SH2 localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-SH2-KDEL
   

   Tags

   PRLSS

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Salsa-BC6-P1A6

   Plasmid

   #202847

   Purpose

   Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6

   Depositor

   Noah Palm

   Article

   Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

   Insert

   EGFP/BC6
   

   Expression

   Mammalian

   Promoter

   Tet Response Element (TRE)

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB30

   Plasmid

   #122247

   Purpose

   Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEE390

   Plasmid

   #149280

   Purpose

   T-DNA encoding TRV2 with gRNA targeting NbPDS3

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_NbPDS3sgRNA:tNos
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSN074

   Plasmid

   #166731

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-NLS
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330A_D10A-1x3

   Plasmid

   #58773

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_Lyn11-STf

   Plasmid

   #182008

   Purpose

   CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   Lyn11-STf
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pED7x26

   Plasmid

   #134813

   Purpose

   Stringent phage propagation reporter containing pIII-16-29-34-TAGA

   Depositor

   Ahmed Badran

   Article

   DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

   Insert

   pIII 16-TAGA 29-TAGA 34-TAGA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   16-TAGA 29-TAGA 34-TAGA

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEE387

    Plasmid

    #149285

    Purpose

    T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_FT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ138A

    Plasmid

    #69280

    Purpose

    Golden Gate entry vector; 8th gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ137A

    Plasmid

    #69279

    Purpose

    Golden Gate entry vector;7th gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ159

    Plasmid

    #69308

    Purpose

    Gateway entry vector with hSpCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9D10A (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Mutation

    D10A mutation; NLS fusion

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSN069

    Plasmid

    #166728

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6_(GLuc)_sgRNA

    Plasmid

    #68422

    Purpose

    Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Gluc sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pG418R-GMRWhite

    Plasmid

    #165883

    Purpose

    G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRU205

    Plasmid

    #167685

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::asCpf1
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRU321

    Plasmid

    #167681

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::asCas9
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRU320

    Plasmid

    #167682

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::asCas9
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. S7_SwaI_Zeo_PacI

    Plasmid

    #128471

    Purpose

    Zeo Resistance gene

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    Zeocin Resistance gene
    

    Expression

    Bacterial

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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