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pX330A-FBL/PITCh

Plasmid

#63671

Purpose

Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pQCXIH-Myc-YAP-5SA

Plasmid

#33093

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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pSLIK-Hygro

Plasmid

#25737

Purpose

3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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HA-HIF1alpha-wt-pBabe-puro

Plasmid

#19365

Depositor

William Kaelin

Publication

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt human cDNA

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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4xM67 pTATA TK-Luc

Plasmid

#8688

Depositor

Jim Darnell

Publication

Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

Insert

4 repeated M67 sites

Use

Luciferase

Expression

Mammalian

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pDest-565

Plasmid

#11520

Purpose

Gateway destination vector for bacterial expression; His,GST tag

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway Cloning

Tags

6xHis and GST

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pIRESneo-FLAG/HA Ago1

Plasmid

#10820

Depositor

Thomas Tuschl

Publication

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 1 (AGO1 Human)

Tags

FLAG and HA

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pSFFV_mNG2(11)1-10

Plasmid

#82610

Purpose

Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

SFFV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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tdTomato-C1

Plasmid

#54653

Purpose

Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Type

Empty backbone

Tags

tdTomato

Expression

Mammalian

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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murine TCR OTI-2A.pMIG II

Plasmid

#52111

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Publication

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

T2A (from Thosea asigna virus)

Expression

Mammalian

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T1

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pCI HA NEDD4

Plasmid

#27002

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pDONR221_EGFP

Plasmid

#25899

Purpose

Gateway entry vector containing EGFP.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

EGFP

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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eIF2a 1

Plasmid

#21807

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Expression

Mammalian

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Publication

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Tags

Flag

Expression

Mammalian

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Publication

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pUCM-AAVS1-TO-hNGN2

Plasmid

#105840

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

Depositor

Michael Ward

Publication

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCDH-EF1-Luc2-P2A-tdTomato

Plasmid

#72486

Purpose

Express Luc2 and tdTomato under EF1 promoter

Depositor

Kazuhiro Oka

Insert

Luc2-P2A-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

EF1

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Publication

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1(+) Flag-His-ATM kd

Plasmid

#31986

Depositor

Michael Kastan

Publication

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM kd (ATM Human)

Tags

Flag and His

Expression

Mammalian

Mutation

3'UTRf 9 nucl/D2870A N2875k. catalytically …

Available since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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