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pSFFV_mNG2(11)1-10

Plasmid

#82610

Purpose

Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

SFFV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pUCM-AAVS1-TO-hNGN2

Plasmid

#105840

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

Depositor

Michael Ward

Publication

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Neurogenin 2 (NEUROG2 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCDH-EF1-Luc2-P2A-tdTomato

Plasmid

#72486

Purpose

Express Luc2 and tdTomato under EF1 promoter

Depositor

Kazuhiro Oka

Insert

Luc2-P2A-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

EF1

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pX330A-FBL/PITCh

Plasmid

#63671

Purpose

Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pHAGE-TO-dCas9-3XmCherry

Plasmid

#64108

Purpose

Expression of Sp dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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murine TCR OTI-2A.pMIG II

Plasmid

#52111

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Publication

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

T2A (from Thosea asigna virus)

Expression

Mammalian

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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deltaNp63alpha-FLAG

Plasmid

#26979

Depositor

David Sidransky

Publication

Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ΔNP63alpha (TP63 Human)

Tags

FLAG

Expression

Mammalian

Mutation

dominant negative isoform of p63; aa 1–587

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pDONR221_EGFP

Plasmid

#25899

Purpose

Gateway entry vector containing EGFP.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

EGFP

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Publication

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pQCXIH-Myc-YAP-5SA

Plasmid

#33093

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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pSLIK-Hygro

Plasmid

#25737

Purpose

3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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eIF2a 1

Plasmid

#21807

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Expression

Mammalian

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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HA-HIF1alpha-wt-pBabe-puro

Plasmid

#19365

Depositor

William Kaelin

Publication

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt human cDNA

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pIRESneo-FLAG/HA Ago1

Plasmid

#10820

Depositor

Thomas Tuschl

Publication

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Argonaute 1 (AGO1 Human)

Tags

FLAG and HA

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pUCmini-iCAP-AAV.CAP-B10

Plasmid

#175004

Purpose

non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

Depositor

Viviana Gradinaru

Publication

Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

Insert

Synthetic construct isolate AAV.CAP-B10 VP1 gene

Use

AAV

Expression

Mammalian

Mutation

7 amino acid substitution between VP1 452 and VP1…

Promoter

p41

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEGFP-C2-NLRP3

Plasmid

#73955

Purpose

Express EGFP-tagged human NLRP3 in mammalian cells

Depositor

Christian Stehlik

Publication

Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV iE

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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tdTomato-C1

Plasmid

#54653

Purpose

Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Type

Empty backbone

Tags

tdTomato

Expression

Mammalian

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_GFP-T1

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pEGFP ZO1

Plasmid

#30313

Depositor

Alan Fanning

Publication

Fanning et al FASEB J. 2002 Nov . 16(13):1835-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Zonula Occludens-1 (TJP1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Unique spliceform*

Available since

Aug. 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCI HA NEDD4

Plasmid

#27002

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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