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Showing: 2041 - 2080 of 5031 results

  1. pLgw EcoDam-V5-ELK1Y158A

    Plasmid
    #98608
    Purpose
    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Insert
    ELK1Y158A (Elk1 Mouse)
    Use
    Lentiviral; Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Mutation
    Promoter
    Heat Shock Minimal Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98608-plasmid-map-sequence-id-189405

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  2. pLexA::nud-1

    Plasmid
    #11339
    Depositor
    Guy Caldwell
    Insert
    nud-1 (nud-1 Nematode)
    Use
    Tags
    LexA DNA Binding Domain
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only

  3. pLexA::lis-1

    Plasmid
    #11338
    Depositor
    Guy Caldwell
    Insert
    lis-1 (lis-1 Nematode)
    Use
    Tags
    LexA DNA Binding Domain
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only

  4. pCambia-dLB-kanR-GFP

    Plasmid
    #223177
    Purpose
    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border
    Depositor
    Andreas Stuermer
    Type
    Empty backbone
    Use
    Ti plasmid for agrobacterium
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    223177-plasmid-map-sequence-id-443110

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  5. ABE7.10-F148A

    Plasmid
    #132946
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    ABE7.10(F148A)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    ABE7.10(F148A)
    Promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    132946-plasmid-map-sequence-id-314827

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  7. AAV-short_BrokenHeart (no BC)

    Plasmid
    #86950
    Purpose
    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.
    Depositor
    Joseph Dougherty, Rob Mitra
    Article
    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    Brokenheart construct
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    86950-plasmid-map-sequence-id-172437

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  8. BE3-hA3A-R128A

    Plasmid
    #132944
    Purpose
    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-hA3A(R128A)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    BE3-hA3A(R128A)
    Promoter
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132944-plasmid-map-sequence-id-314824

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  9. BE3-hA3A-Y130F

    Plasmid
    #132945
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-hA3A(Y130F)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    BE3-hA3A(Y130F)
    Promoter
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132945-plasmid-map-sequence-id-314826

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  10. pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

    Plasmid
    #215026
    Purpose
    Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
    Depositor
    Joanna Wysocka
    Article
    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4X E-box mutations within Coordinator motifs
    Promoter
    SV40
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    215026-plasmid-map-sequence-id-425844

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  12. BE3-YE1

    Plasmid
    #132943
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-(W90Y-R126E)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    BE3-(W90Y-R126E)
    Promoter
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132943-plasmid-map-sequence-id-314816

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  13. JARID1BA

    Plasmid
    #74646
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JARID1BA (KDM5B Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    Leu101-(Gly)4-Ala374 deletion mutant
    Promoter
    PH
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. JARID1BA

    Plasmid
    #74648
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JARID1BA (KDM5B Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    Leu101-(Gly)4-Ala374 deletion mutant
    Promoter
    PH
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pNIC28-hKdm2b-CxxC-PHD

    Plasmid
    #49216
    Purpose
    Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.
    Depositor
    Rob Klose
    Insert
    Kdm2b (KDM2B Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    49216-plasmid-map-sequence-id-72771

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  23. DDI1

    Plasmid
    #32867
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DDI1 (DDI1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    amino acids 239-367 only
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

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Showing: 2041 - 2080 of 5031 results