Addgene

We narrowed to 2,327 results for: 683

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pYPQ141-ZmUbi-RZ-Aac.4

Plasmid

#158406

Purpose

Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin 1

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-2xOsh2 PH domain [short]

Plasmid

#58829

Purpose

Expresses GFP-Tagged Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2xOsh2 PH domain [short] (OSH2 Budding Yeast)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-MyD88-FW/AA

Plasmid

#12289

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

F285A/W286A

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pMLS236

Plasmid

#73684

Purpose

SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSP568

Plasmid

#139493

Purpose

Insertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xMS2sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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Lifeact-rsLOV2 pcDNA3.1(+)

Plasmid

#107882

Purpose

Mammalian expression of Lifeact-rsLOV2

Depositor

Stefan Hell

Article

Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

Insert

rsLOV2

Use

Tags

Lifeact

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pOPTN1-55-EGFP

Plasmid

#68838

Purpose

deleted OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-55 only

Promoter

CMV

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pUAS-MmTFP13xmyc

Plasmid

#86371

Purpose

membrane targeted Tfp1 expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

mTfp1

Use

Zebrafish

Tags

3xMyc and GAP43 membrane targeting

Expression

Mutation

Promoter

UAS

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pVW284

Plasmid

#139494

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 PP7∆FG-GFPenvy tCYC1

Use

Tags

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVW300

Plasmid

#139495

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pTEF PP7∆FG-GFPenvy tCYC1

Use

Tags

Expression

Yeast

Mutation

∆FG

Promoter

pTEF

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-I179N

Plasmid

#12295

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

I179N

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
EHMT1 (3HNA)

Plasmid

#25305

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

EHMT1_07 (EHMT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

C-terminal domain, residues 951-1235

Promoter

Available since

Aug. 16, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pTRE/WT SP with 5'UTR

Plasmid

#89330

Purpose

Inducible expression of WT spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet-responsive P tight

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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MAT2B

Plasmid

#39080

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MAT2B (MAT2B Human)

Use

Tags

Tev His Flag

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pYPQ141-ZmUbi-RZ-Aa3.8.5

Plasmid

#158407

Purpose

Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin 1

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-P200H

Plasmid

#12288

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

P200H

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pKS2311

Plasmid

#37835

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2cpl-1W32A;Y35A::YFP (cpl-1 Nematode)

Use

Tags

YFP

Expression

Worm

Mutation

W32A;Y35A

Promoter

nhx-2

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pJT303_SNR52_sgRNA_Can1-3

Plasmid

#145066

Purpose

Expresses sgRNA targeting Can1-3 site

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

Can1-3 sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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Lifeact-rsLOV1 pcDNA3.1(+)

Plasmid

#107881

Purpose

Mammalian expression of Lifeact-rsLOV1

Depositor

Stefan Hell

Article

Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

Insert

rsLOV1

Use

Tags

Lifeact

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge