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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Publication

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Publication

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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MDM2p-Mdm2-YFP

Plasmid

#53962

Purpose

Expresses YFP tagged Mdm2 from MDM2 promoter

Depositor

Uri Alon, Galit Lahav

Publication

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MDM2 (MDM2 Human)

Tags

EYFP

Expression

Mammalian

Promoter

MDM2

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLOVE-Nmyc

Plasmid

#15951

Depositor

Miguel Ramalho-Santos

Publication

Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Available since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
IRES-GFP FoxG1

Plasmid

#14935

Depositor

Joan Massague

Publication

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FoxG1 (FOXG1 Human)

Tags

IRES-GFP

Expression

Mammalian

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP delta

Plasmid

#12559

Depositor

Peter Johnson

Publication

McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)

Expression

Mammalian

Mutation

S2G introduced during cloning

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pAAV-fSST-RFP

Plasmid

#22913

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu somatostatin promoter driving DsRed 2

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Publication

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDTA-TK

Plasmid

#22677

Depositor

Robert Benezra

Publication

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

DTA and TK

Use

Mouse Targeting

Mutation

pDTA-TK dual selection plasmid for targeting vect…

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBa.TfR.GFP

Plasmid

#45060

Depositor

Gary Banker, Marvin Bentley

Publication

Burack et al Neuron. 2000 May;26(2):465-72.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TfR (TFRC Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta Actin

Available since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCMV-Caspase1-flag

Plasmid

#21142

Depositor

Junying Yuan

Publication

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

caspase-1 (Casp1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCDNA3 Flag Jnk2a2

Plasmid

#13755

Depositor

Roger Davis

Publication

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Jnk2a2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p11-LacY-wtx1

Plasmid

#69056

Purpose

The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

Depositor

Huimin Zhao

Publication

Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

Inserts

ccdB (ccdB )
lacY (lacY )

Tags

A177C

Promoter

pBAD and pLac

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pLOVE-GFP

Plasmid

#15949

Depositor

Miguel Ramalho-Santos

Publication

Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

Insert

Green Fluorescent Protein

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Available since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-GFAP104-mCherry

Plasmid

#58909

Purpose

AAV-mediated expression of mCherry under the GFAP promoter

Depositor

Edward Boyden

Publication

Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

Has service

AAV5

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCDH-Flag-c-Myc

Plasmid

#102626

Purpose

Expresses c-Myc in mammalian cells

Depositor

Hening Lin

Publication

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

MYC proto-oncogene, bHLH transcription factor (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Publication

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Expression

Bacterial

Promoter

pBAD

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Publication

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has service

AAV8

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Promoter

CAG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Publication

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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T/Brachyury-eGFP Rex Neo

Plasmid

#21222

Depositor

Mark Mercola

Publication

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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