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pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP delta

Plasmid

#12559

Depositor

Peter Johnson

Article

McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

Insert

CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)

Expression

Mammalian

Mutation

S2G introduced during cloning

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pLOVE-Nmyc

Plasmid

#15951

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Available Since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-fSST-RFP

Plasmid

#22913

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu somatostatin promoter driving DsRed 2

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDTA-TK

Plasmid

#22677

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

DTA and TK

Use

Mouse Targeting

Mutation

pDTA-TK dual selection plasmid for targeting vect…

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pBa.TfR.GFP

Plasmid

#45060

Depositor

Gary Banker, Marvin Bentley

Article

Burack et al Neuron. 2000 May;26(2):465-72.

Insert

TfR (TFRC Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Caspase1-flag

Plasmid

#21142

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

caspase-1 (Casp1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a2

Plasmid

#13755

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pLOVE-GFP

Plasmid

#15949

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

Insert

Green Fluorescent Protein

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Available Since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

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p11-LacY-wtx1

Plasmid

#69056

Purpose

The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

Depositor

Huimin Zhao

Article

Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

Inserts

ccdB (ccdB )
lacY (lacY )

Tags

A177C

Promoter

pBAD and pLac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-mCherry

Plasmid

#58909

Purpose

AAV-mediated expression of mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

Has Service

AAV5

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Expression

Bacterial

Promoter

pBAD

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-c-Myc

Plasmid

#102626

Purpose

Expresses c-Myc in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

MYC proto-oncogene, bHLH transcription factor (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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T/Brachyury-eGFP Rex Neo

Plasmid

#21222

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Promoter

CAG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe 12S E1A

Plasmid

#18742

Depositor

Scott Lowe

Article

Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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PCDNA3 Flag p38 alpha (agf)

Plasmid

#20352

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Replace Thr 180 and Tyr 182 with Ala and Phe resp…

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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