We narrowed to 10,453 results for: T7

1. pETA_21 tRNA genes

   Plasmid

   #242431

   Purpose

   Expresses 21 tRNAs. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site as well as a T7 terminator downstream of each tRNA gene.

   Depositor

   Sebastian Maerkl

   Article

   Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

   Insert

   21 tRNA genes
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRII-mogat3b in situ probe

   Plasmid

   #232136

   Purpose

   zebrafish mogat3b anti-sense in situ probe, linearize with BamHI, T7 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   mogat3b
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6/T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSETA-qmTQ2-glucose

   Plasmid

   #225252

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-glucose
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETA-qmTQ2-cAMP

   Plasmid

   #225249

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-cAMP
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL25_Cas9-His Δcys E1207C

   Plasmid

   #206286

   Purpose

   Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1207C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDL23_Cas9-His Δcys E945C

   Plasmid

   #206284

   Purpose

   Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E945C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDL24_Cas9-His Δcys E1026C

   Plasmid

   #206285

   Purpose

   Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1026C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDL22_Cas9-His Δcys E532C

   Plasmid

   #206283

   Purpose

   Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET23PI-Sce ESARC D218A

   Plasmid

   #195608

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

   Depositor

   Frederick Gimble

   Article

   Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T928C, T929C, A942C

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7PI-Sce ESARC D326A

    Plasmid

    #195609

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T3593C, A3601C, T3602G

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET23PI-Sce ESARC

    Plasmid

    #195607

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

    Promoter

    T7

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEU3-NII-HxHLICNot

    Plasmid

    #140183

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    12xHis

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19_TTE1564_UUCG-loop

    Plasmid

    #133581

    Purpose

    T7-transcription template for a P2-deletion mutation of the Tte preQ1- riboswitch

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    P2-deletion mutant of Tte preQ1-riboswitch
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    replacement of riboswitch nucleotides U6 through …

    Promoter

    T7

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET OCaMP

    Plasmid

    #224932

    Purpose

    Expresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope)

    Depositor

    Alexander Lohman

    Article

    Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

    Insert

    OCaMP
    

    Use

    Tags

    6xHis, RSET peptide, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 28, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pXT7

    Plasmid

    #32995

    Depositor

    Sergei Sokol

    Type

    Empty backbone

    Use

    Xenopus rna expression

    Tags

    globin 3'UTR and globin 5' UTR

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

16. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Use

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-HsSENP1

    Plasmid

    #71465

    Purpose

    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET28a T7pCONS TIR-2 sfGFP

    Plasmid

    #154464

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMH48

    Plasmid

    #131861

    Purpose

    Produces holo-Cph1* in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

    Inserts

    Cph1(1-514,Y263F)-RGD-His6-Cys
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a T7pCONS TIR-1 sfGFP

    Plasmid

    #154463

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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