We narrowed to 2,758 results for: ada.2

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pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

Plasmid

#111387

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
ChETA

Use

AAV

Tags

HA and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_N

Plasmid

#209553

Purpose

pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

TadA-8e(2-76)-2xFRB

Expression

Mammalian

Promoter

pCMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTK305

Plasmid

#66812

Purpose

IVT vector for Fluc mRNA

Depositor

Ron Weiss

Article

Andries et al J Control Release. 2015 Sep 2. pii: S016

Insert

Firefly luciferase

Use

Luciferase; Mrna ivt

Promoter

T7

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pTPH418b

Plasmid

#185725

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTPH405c

Plasmid

#185726

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C) single PAM linker

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25_IRES-mCherry

Plasmid

#198386

Purpose

RNF25 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF14_IRES-mCherry

Plasmid

#198384

Purpose

RNF14 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF14 (RNF14 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFLAG-ZYG11B

Plasmid

#110550

Purpose

expresses a FLAG-tagged human ZYG11B protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11B (ZYG11B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pFLAG-ZYG11A

Plasmid

#110551

Purpose

expresses a FLAG-tagged human ZYG11A protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11A (ZYG11A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEF6 rglut1 flag complete

Plasmid

#89571

Purpose

rglut1 flag complete

Depositor

Jeffrey Rathmell

Article

Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

Insert

rglut1 flag complete (Slc2a1 Rat)

Tags

Exo Facial

Expression

Mammalian

Promoter

EF-1a

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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EC.SurA.TYB1.a172-274.wt

Plasmid

#15934

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

Peptidyl prolyl isomerase domain 1 of SurA (surA E. coli)

Tags

intein

Expression

Bacterial

Mutation

wild type

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA_T

Plasmid

#147634

Purpose

Insect Expression of HsTNRC6C_1207-1609-M2WA

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6C_1207-1609-M2WA (TNRC6C Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609_R

Plasmid

#147410

Purpose

Insect Expression of HsTNRC6C_1207-1609

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6C_1207-1609 (TNRC6C Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGPN

Plasmid

#187482

Purpose

Expression vector - GFP

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

EGFP

Use

Lentiviral

Promoter

EFS

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Omicron.BA.5-RBD

Plasmid

#213478

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/Omicron.BA.5 RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-2P-OMICRON

Plasmid

#180593

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Article

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

Plasmid

#111395

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
DTR-EGFP

Use

AAV

Tags

EGFP and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_dTAG-KANSL3-targeting

Plasmid

#170755

Purpose

Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

BSD-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

BSD-P2A-2xHA-dTAG

Mutation

Phenylalanine 36 to valine in FKBP12

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-CLCa

Plasmid

#196912

Purpose

Visualization of clathrin-coated pit formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

Insert

Clathrin-Coated light chain

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ262m3

Plasmid

#149556

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)

Use

CRISPR

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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