We narrowed to 6,084 results for: nls

1. pPHR-iRFP713-NLS-VP16

   Plasmid

   #103823

   Purpose

   Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-iRFP713-VP16 (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VP16: G126T (silent, G42G)

   Promoter

   CMV

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. ptagBFP-LacI-NLS-VP16

   Plasmid

   #103837

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   tagBFP-LacI-NLS-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

   Plasmid

   #47453

   Purpose

   epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE (N136,Grm2,C63)-cib1
   

   Use

   AAV; Tale

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLR5-CAG-NLS-tdiRFP670

   Plasmid

   #82621

   Purpose

   Expresses NLS-tdiRFP670 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pattB-nsyb-MKII::nlsLexADBDo

   Plasmid

   #64725

   Purpose

   generating transgenic TRIC flies

   Depositor

   Liqun Luo

   Article

   Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

   Insert

   MKII::nlsLexADBDo
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-MCS-YFP-NLS

   Plasmid

   #145274

   Purpose

   Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MCS and YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2-7xTcf-NLS-ONL-CP

   Plasmid

   #65716

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2-7xTcf-NLS-YNL-CP

   Plasmid

   #65714

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-NLS-YFP-CCTd

   Plasmid

   #184325

   Purpose

   Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)

   Depositor

   Xiaodong Liu

   Article

   Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

   Insert

   CCTd (CACNA1D Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. RPL25NLS-GFP-PRA (102)

    Plasmid

    #24041

    Depositor

    Michael Rout

    Insert

    RPL25 (RPL25 Budding Yeast)
    

    Use

    Tags

    EGFP and PRA

    Expression

    Yeast

    Mutation

    Only bp 1-135 (aa 1-45) of RPL25

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pT2-7xTcf-NLS-CNL-CP

    Plasmid

    #65715

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol1-uas-mAKT-2a-nlstdTomato

    Plasmid

    #67706

    Purpose

    Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    mAKT-2A-nlstdTomato
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

    Plasmid

    #172195

    Purpose

    AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

    Depositor

    Garrett Kaas

    Article

    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

    Insert

    HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
    

    Use

    AAV and Mouse Targeting; Tale

    Tags

    HA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    transcription regulatory domain removed

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pattB-nsyb-MKIIK11A::nlsLexADBDo

    Plasmid

    #64726

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    MKIIK11A::nlsLexADBDo
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ptagBFP-TetR-NLS-VP16

    Plasmid

    #103835

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-TetR-NLS-VP16 (tetR )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid

    #89743

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi dark-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid

    #89742

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi lit-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid

    #135355

    Purpose

    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mPA-GFP-PCNA-19-SV40NLS-4

    Plasmid

    #57154

    Purpose

    Localization: Nucleus, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pAAV-S5E8-dTom-nlsdTom

    Plasmid

    #135643

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E8 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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