We narrowed to 6,501 results for: nls
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Plasmid#50836PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddGFP B
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP2372
Plasmid#127578PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FKBP-ICUE3-NLS
Plasmid#138255PurposeFRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP-ICUE3-NLS
TagsFKBP, Nuclear localization signal (NLS), cpVenus[…ExpressionMammalianPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPS1889
Plasmid#1361DepositorPublicationInsertNLS-ECFP-EYFP
ExpressionYeastAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddRFP A
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
864 pSG5L HA NLS PTEN
Plasmid#10933DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPTEN (PTEN Human)
TagsHA and NLSExpressionMammalianAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-Flag-SpCas9-HF1 (without sgRNA)
Plasmid#92102PurposeExpression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926APromoterCbhAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-USP7
Plasmid#99393PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7DepositorInsertUSP7 (USP7 Human)
TagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-puro-NLS-GFP
Plasmid#185473PurposeMammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMammalianPromoterCMVAvailable sinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pNB330
Plasmid#60147PurposeDisintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)DepositorInsertNLuc
TagsNuclear localization signal (NLS)ExpressionYeastPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10
Plasmid#219701PurposeLevel 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1DepositorInsertpAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
ExpressionPlantAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-NLS-mEGFP
Plasmid#105527PurposeT7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signalDepositorInsertNucleoplasmin
UseIn vitro transcriptionTagsmEGFPMutationNLS of nucleoplasmin (encoding amino acids KRPAAT…PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MTK3_008
Plasmid#123723PurposeEncodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-VP16-EL222
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry
Plasmid#68279PurposeSKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherryDepositorInsertFar1DS NLS mCherry
TagsmCherryExpressionYeastPromoterRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED73 pRS304 pRPS2-SteDS-NLS-mCitrine
Plasmid#68278PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrineDepositorInsertSte7DS NLS mCitrine
TagsmCitrineExpressionYeastPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-BFP-NLS-P2A-RSV P-1xALFATag@72AA
Plasmid#246864PurposeExpress BFP-NLS-P2A-RSV P-1xALFATag@72AA (Pexo-ALFATag) for RSV imaging.DepositorInsertBFP-NLS-P2A-RSV P-1xALFATag@72AA
UseLentiviralTagsBFP-NLSExpressionMammalianMutationRSV phosphoprotein (P) contains a single ALFA Tag…PromoterSFFVAvailable sinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-miniTurbo-YFP-NLS
Plasmid#127370PurposeBinary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plantsDepositorInsertminiTurbo (BirA mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB_SBE4:mScarlet-I-NLS
Plasmid#154961Purposeencodes transcriptional reporter of SMAD2/3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet
UsePiggybacTagsNLS-PESTExpressionMammalianAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-Flag-HA
Plasmid#101766PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA & Flag-tag fusion proteinDepositorTypeEmpty backboneTagsFlag-HAExpressionMammalianAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-1BPNLS-Cas9-1BPNLS-2AGFP
Plasmid#87109PurposeBPNLS-Cas9-BPNLS-2AGFP expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert1BPNLS-Cas9-1BPNLS-2A-EGFP
UseCRISPRTags3xFLAG (on Cas9)ExpressionMammalianPromoterCAGAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by