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We narrowed to 6,447 results for: nls

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Showing: 1021 - 1040 of 6447 results

  1. BNLS

    Plasmid
    #50836
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP B
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50836-plasmid-map-sequence-id-76280

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  2. RANLS

    Plasmid
    #50843
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50843-plasmid-map-sequence-id-76292

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  4. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  5. TK4-NLS-EGFP

    Plasmid
    #239046
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Marius Wernig
    Article
    Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2
    Inserts
    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2 and c-myc-NLS
    Expression
    Mammalian
    Promoter
    CAG and EF1a
    Available Since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    239046-plasmid-map-sequence-id-473412

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  8. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

    Plasmid
    #219701
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219701-plasmid-map-sequence-id-435731

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  11. pGEMHE-NLS-mEGFP

    Plasmid
    #105527
    Purpose
    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal
    Depositor
    Melina Schuh
    Article
    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31
    Insert
    Nucleoplasmin
    Use
    In vitro transcription
    Tags
    mEGFP
    Mutation
    NLS of nucleoplasmin (encoding amino acids KRPAAT…
    Promoter
    T7
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    105527-plasmid-map-sequence-id-201844

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  15. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid
    #127370
    Purpose
    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127370-plasmid-map-sequence-id-246351

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  16. pAAVss-G1B3-nlsCre-WPRE-miR124T-SYNpolyA

    Plasmid
    #245080
    Purpose
    CRE recombinase with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Nicole Déglon
    Insert
    nls-Cre
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    245080-plasmid-map-sequence-id-488835

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  17. SIN-cPPT-G1B3-nlsCre-WPRE-miR124T

    Plasmid
    #245072
    Purpose
    CRE recombinase with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Nicole Déglon
    Insert
    nls-CRE
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245072-plasmid-map-sequence-id-488269

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  20. pCSnERT(#186)

    Plasmid
    #184057
    Purpose
    to express cyclofen-inducible fusions in mammalian cells or fish eggs
    Depositor
    Sophie Vriz
    Insert
    nls-ERT2
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184057-plasmid-map-sequence-id-365952

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Showing: 1021 - 1040 of 6447 results