We narrowed to 59,461 results for: %s

1. pBsVchCAST-pyrE

   Plasmid

   #203813

   Purpose

   Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMALp2x-CARP-1

   Plasmid

   #41727

   Depositor

   Koji Okamoto

   Article

   Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   clam ADP-ribosylating protein-1
   

   Use

   Bacterial subcloning

   Mutation

   deleted 5'-UTR nucleotides 1-64

   Promoter

   pMB1

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pET41sIII_GST-ALC1

   Plasmid

   #173932

   Purpose

   Bacterial expression of ALC1

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1 (CHD1L Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMH-SFB-BRCA2

   Plasmid

   #99395

   Purpose

   Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2

   Depositor

   Junjie Chen, Michael Huen

   Article

   Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

   Insert

   BRCA2 (BRCA2 Human)
   

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV3Tag8li-hADARB2-3xFLAG

   Plasmid

   #165470

   Purpose

   Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA2-DDB1

   Plasmid

   #19909

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   Damage-specific DNA binding protein 1 (DDB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   please see depositor comment below

   Available Since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-Nkx2-1/Puro

   Plasmid

   #31271

   Depositor

   Tyler Jacks, Monte Winslow

   Article

   Winslow et al Nature. 2011 May 5. 473(7345):101-4.

   Insert

   Nkx2-1* (Nkx2-1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

   Plasmid

   #155125

   Purpose

   Tol2 vector containing Cre-inducible QFGal4-switch

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QFGal4-switch
   

   Use

   Cre/Lox; Zebrafish expression

   Promoter

   Ubi

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQFlag-USP7 WT puroR

   Plasmid

   #46751

   Purpose

   Retroviral vector that expresses wild type Flag-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid

    #84239

    Purpose

    Expresses ABA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    ABI-tagBFP-Sp dCas9
    PYL1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

    Plasmid

    #163024

    Purpose

    Expression of CRISPRi with U6-gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 polyA, and myc NLS

    Expression

    Mammalian

    Promoter

    hEF-1a

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)

    Plasmid

    #85039

    Purpose

    For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    RFP-P2A-tTA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVTNT PINK1 N-myc

    Plasmid

    #13313

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBMN mEGFP-NDP52

    Plasmid

    #188785

    Purpose

    For mammalian expression of NDP52 tagged with mEGFP

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ALFA-bArr1

    Plasmid

    #201495

    Purpose

    Expression of the beta-arrestin1 containing a N-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    ALFA-bArr1 (ARRB1 Human)
    

    Tags

    ALFA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-NUP188

    Plasmid

    #159893

    Purpose

    Expresses NUP188 in mammalian cells

    Depositor

    Kozo Tanaka

    Article

    Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

    Insert

    NUP188 (NUP188 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    To avoid NotI site, first three amino acids are d…

    Promoter

    CMV

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ALFA-bArr2

    Plasmid

    #201497

    Purpose

    Expression of the beta-arrestin2 containing a N-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    ALFA-bArr2 (ARRB2 Human)
    

    Tags

    ALFA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR-HOT-Clover-BlastR

    Plasmid

    #138569

    Purpose

    Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    Clover-BlastR
    

    Use

    CRISPR

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pv6_TAF3_2xPhd

    Plasmid

    #179399

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3_2xPhd

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    TAF3_2xPhd
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. amilCP_Orange chromoprotein

    Plasmid

    #117850

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP_Orange
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    64V,65G, BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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