We narrowed to 12,202 results for: 112

1. pcDNA3.1 TAP DHX36

   Plasmid

   #53547

   Purpose

   mammalian expression of DHX36

   Depositor

   Kathleen Collins

   Article

   Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   TAP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIA33

   Plasmid

   #120803

   Purpose

   E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

   Depositor

   David S. Weiss, Craig Ellermeier

   Article

   Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

   Inserts

   deactivated nuclease dCas9, codon-optimized for C. difficile
   single guide RNA targeting red fluorescent protein
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Base-pairing region targets red fluorescent prote…

   Promoter

   Pgdh and Pxyl

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CXCR6-DuET

   Plasmid

   #213223

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CXCR6 (CXCR6 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-EphB2

   Plasmid

   #200982

   Purpose

   Mammalian expression plasmid for myc-tagged EphB2

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB2 (EPHB2 Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-RHO-IntC-dCas9C-VPR-polyA

   Plasmid

   #166692

   Purpose

   Express C-terminal part of split dCas9-VPR

   Depositor

   Stylianos Michalakis

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Insert

   IntC-dCas9C-VPR
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   short human rhodopsin promoter

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CXCR2-DuET

   Plasmid

   #213219

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CXCR2 (CXCR2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTarget

   Plasmid

   #214052

   Purpose

   Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Target sequence
   

   Use

   Target rna plasmid

   Expression

   Bacterial

   Promoter

   trc promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHochTypeIII

   Plasmid

   #214039

   Purpose

   Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Cas10
   

   Use

   CRISPR

   Mutation

   WT

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUW-tetO-BCL6

   Plasmid

   #139816

   Purpose

   doxycycline-inducible expression of Human BCL6 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   BCL6 (BCL6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   T190A, E241G, L314M- please see depositor comments

   Promoter

   TRE-mCMV

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. hRAP-pEZT-BM

    Plasmid

    #177986

    Purpose

    Expression human RAP containing a C-terminal 6xHis tag in mammalian cells

    Depositor

    Lora Hooper

    Article

    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

    Insert

    LDL receptor related protein associated protein 1 (LRPAP1 Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.0-TERT G228A

    Plasmid

    #84926

    Purpose

    luciferase reporter for mutant TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    G228A

    Promoter

    TERT

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GAL1-DuET

    Plasmid

    #213248

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GAL1 (GALR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PLX_307 FDX1

    Plasmid

    #184495

    Purpose

    Lentivirus expression for FDX1

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-UbcP-Myc-YWAA-pGK-HYG

    Plasmid

    #107377

    Purpose

    Lentiviral expression of myc-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    UbcP

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 2xmyc-RAB10

    Plasmid

    #164631

    Purpose

    Expresses RAB10 in mammalian cells with a 2xmyc tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    Ras-related protein Rab-10 (RAB10 Human)
    

    Tags

    2xmyc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.0-TERT G250A

    Plasmid

    #84925

    Purpose

    luciferase reporter for mutant TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    G250A

    Promoter

    TERT

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pSLIK_hygro_BirA_NRF2

    Plasmid

    #136539

    Purpose

    Lentiviral expression vector for an inducible BirA-NRF2

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA-NRF2 (NFE2L2 Human)
    

    Use

    Lentiviral; Destination vector for gateway cloning

    Expression

    Mammalian

    Available Since

    June 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUW-tetO-Bcl11a

    Plasmid

    #139809

    Purpose

    doxycycline-inducible expression of human Bcl11a in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    BCL11a (BCL11A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-Rheb (Q64L)-pcw107

    Plasmid

    #64607

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RHEB (RHEB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    Q64L

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLenti6.3/TO/V5-DEST-IGFBP2-Clover

    Plasmid

    #191008

    Purpose

    Lentiviral expression vector for IGFBP2 with a fluorescent Clover tag

    Depositor

    Johanna Ivaska

    Article

    Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

    Insert

    Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
    

    Use

    Lentiviral

    Tags

    Clover

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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