164,853 results

1. PCS4 3XFLAG-PPARgamma1

   Plasmid

   #78769

   Purpose

   To overexpress PPARgamma1 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

   Insert

   PPAR_1 (Pparg Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-23:TJP1-mEGFP

   Plasmid

   #87429

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   March 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pUC19-TgKI-MCS-tdTomato-polyA

   Plasmid

   #174022

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   tdTomato
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

   Plasmid

   #62044

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   LAP2beta (Tmpo Rat)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-EFS-LSL-nlsGFP-shRNA

   Plasmid

   #175584

   Purpose

   Conditional express shRNA with control of LoxP

   Depositor

   Christian Wolfrum

   Article

   Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

   Type

   Empty backbone

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQF

   Plasmid

   #48095

   Purpose

   Cumate-inducible expression of 3xFLAG-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-UBR5

   Plasmid

   #52050

   Purpose

   CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

   Depositor

   Darren Saunders

   Article

   Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

   Insert

   UBR5 (UBR5 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC1-CMV-mito-DAAO

   Plasmid

   #141132

   Purpose

   Expresses DAAO with mitochondrial localization signal in mammalian cells

   Depositor

   Vsevolod Belousov

   Insert

   mito-DAAO
   

   Use

   Tags

   2 mitochondrial localization signals and 7xHis

   Expression

   Mammalian

   Mutation

   deleted DAAO amino acids 367-368

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCold I-Hero45

   Plasmid

   #187931

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero45

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero45 (SERBP1 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pE-35S-miniSdd7-nCas9-PBE-GmPPO2

    Plasmid

    #204855

    Purpose

    For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI-mScarlet
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    35S, AtU6-26p

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNF02-mJuniper

    Plasmid

    #219398

    Purpose

    Constitutive expression of mJuniper in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mJuniper
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. human p53-(82-393)

    Plasmid

    #24867

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 82-393

    Promoter

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pEGFPC1-hVAP-A

    Plasmid

    #104447

    Purpose

    Expression of human VAP-A fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. cycLuc_TEVS

    Plasmid

    #119207

    Purpose

    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with TEVs cleavage site
    

    Use

    Luciferase

    Tags

    6xHis and AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCutamp

    Plasmid

    #140632

    Purpose

    Plasmid-curing in Escherichia coli by targeting the AmpR promoter

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRC-CMV-Rev1b

    Plasmid

    #164443

    Purpose

    Encodes HIV-1 Rev accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PHR-GFP-FUSN-VP16

    Plasmid

    #183933

    Purpose

    Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-FUSN-VP16 (CRY2, UL48 Mustard Weed, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNCS-mClover3

    Plasmid

    #74236

    Purpose

    Constitutive expression of mClover3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Has Service

    Cloning Grade DNA

    Insert

    mClover3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST-RH▲-MNase

    Plasmid

    #136292

    Purpose

    Expression of catalytic inactive RNase H fused to micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    RNase HI-D10R-E48R and MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge