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We narrowed to 13,839 results for: CRISPR-Cas9

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Showing: 10401 - 10420 of 13839 results

  10. pLEX307_CR-PDHA2S291A/S293A

    Plasmid
    #115205
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115205-plasmid-map-sequence-id-232427

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  12. HCP4

    Plasmid
    #166106
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166106-plasmid-map-sequence-id-330381

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  13. HCP6

    Plasmid
    #166108
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166108-plasmid-map-sequence-id-330318

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  17. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  19. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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Showing: 10401 - 10420 of 13839 results