We narrowed to 59,318 results for: %s

1. AsCas12a-DR-cr in PBCSK-

   Plasmid

   #126640

   Purpose

   Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   AsCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. dmBACCS2NS-IRES-dOrai-IRES-GFP

   Plasmid

   #72895

   Purpose

   Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2NS-IRES-dOrai-IRES-GFP
   

   Tags

   IRES-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS1543

   Plasmid

   #205963

   Purpose

   NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH Cascade with His14-tagged Csf3
   

   Tags

   14xHis on Csf3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV.U6.gDNMT1.Cas9-T2A-GFP

   Plasmid

   #170362

   Purpose

   Plasmid used for Crispr/cas9 based disruption of human DNMT1.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Available Since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CDK4-luciferase full length (2135)

   Plasmid

   #86656

   Purpose

   Luciferase reporter containing CDK4 promoter

   Depositor

   Jaime Modiano

   Article

   Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

   Insert

   CDK4 promoter (CDK4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   2120 base pairs including up to coding nucleotide…

   Promoter

   CDK4

   Available Since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS1871

   Plasmid

   #205976

   Purpose

   U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH DR-BpiI-DR
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS1862

   Plasmid

   #205975

   Purpose

   FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5 from JAGBLH010000220, Cas8-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

   Plasmid

   #107318

   Purpose

   Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUF1-dCas9-Sir2a

   Plasmid

   #122510

   Purpose

   Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.

   Depositor

   Lubin Jiang

   Article

   Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

   Insert

   dCas9-Sir2a
   

   Use

   CRISPR

   Tags

   3*FLAG

   Mutation

   dCas9(D10A,H840A)

   Promoter

   PfHsp86

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FMR1-E17C-3xFLAG

    Plasmid

    #157784

    Purpose

    donor plasmid for C-terminal FLAG tag fusion of human FMR1

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1-FLAG (FMR1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-T7-hOsa1

    Plasmid

    #17986

    Depositor

    Naoko Tanese

    Article

    Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

    Insert

    Osa1 (ARID1A Human)
    

    Tags

    T7 epitope

    Expression

    Mammalian

    Mutation

    Missing 5' end

    Available Since

    Aug. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3-myc3-FBW5 delta F

    Plasmid

    #19912

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    F-box and WD repeat domain containing 5 delta F (FBXW5 Human)
    

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    deleted aa1-42 (F-box)

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH4169

    Plasmid

    #200108

    Purpose

    Prig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    snb-1 (snb-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Prig-3

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. tsPurple chromoprotein promoter-less

    Plasmid

    #118007

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBsVchCAST

    Plasmid

    #203812

    Purpose

    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

    Depositor

    Sheng Yang

    Article

    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FOR human NANOS3 TALEN

    Plasmid

    #59722

    Purpose

    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

17. REV human NANOS3 TALEN

    Plasmid

    #59723

    Purpose

    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. SpeCas9-HF

    Plasmid

    #192141

    Purpose

    Expresses highly specific SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9-HF
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    SpeCas9-HF (R247A, N415A, S421A)

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHS1517

    Plasmid

    #205980

    Purpose

    FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH
    

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. attB-mCh-Poly(A)

    Plasmid

    #65525

    Purpose

    Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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