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pLV Tmem161a-V5

Plasmid

#175149

Purpose

Lentiviral expression of mouse Tmem161a-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem161a (Tmem161a Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCI Flag Smad3 LC EPSM

Plasmid

#27038

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; T179V S204A S208A S213A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pAV114

Plasmid

#63181

Purpose

pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 S204A

Plasmid

#27114

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

S204A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPV-0.8k-Venus-WPRE

Plasmid

#156399

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha-HA-TFAP4

Plasmid

#110158

Purpose

Lentiviral vector for expression of human TFAP4 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-Beclin1 (1-242)

Plasmid

#24401

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 1-242 only.

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pGEMHE-POLArISact

Plasmid

#164970

Purpose

T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Use

In vitro transcription

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV V5-Tmem214

Plasmid

#175153

Purpose

Lentiviral expression of V5-tagged mouse Tmem214

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem214 (Tmem214 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCI Flag Smad3 NL EPSM

Plasmid

#27006

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226; T179V S204A S208A S213A

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pMIG-Flag-RIPK1-D324A

Plasmid

#102678

Purpose

retroviral expression plasmid for RIPK1-D324A

Depositor

Hasam Habelhah

Article

Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

Insert

RIPK1 (RIPK1 Human)

Use

Retroviral

Tags

Flag and IRES-GFP

Expression

Mammalian

Mutation

CHANGE Glutamate 324 to Alanine

Promoter

unknown

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcFLAG-CSK-R107E

Plasmid

#74503

Purpose

Overexpression of CSK R107E mutant in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Tags

FLAG

Expression

Mammalian

Mutation

R107E, K222R

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pFX-β-tubulin-iRFP

Plasmid

#175830

Purpose

Express iRFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-UVRAG CC

Plasmid

#24400

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

UVRAG (UVRAG Human)

Tags

His

Expression

Bacterial

Mutation

Contains on the CC domain, amino acids 200-270

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
1120 pBabe puroL HA FKHR H215R

Plasmid

#9026

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR H215R (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H215R

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-Fah_W

Plasmid

#163087

Purpose

expressing mouse wild type FAH in mammalian cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Insert

Fah_W

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pUPD2 PhiC31 integrase (GB1496)

Plasmid

#160578

Purpose

Streptomyces phage PhiC31 integrase, complete CDS

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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SNAP-MIDAS

Plasmid

#171604

Purpose

Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)

Tags

His6 tag and SNAP tag

Expression

Bacterial

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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