We narrowed to 1,229 results for: Aspa
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Plasmid#106462PurposeExpresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W(N163,242Q)
TagsPAExpressionBacterial and YeastMutationchanged Asparagines at positions 163 and 242 to G…PromoterYBR139WAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-T7-Ntx-K49
Plasmid#58715PurposeExpresses in Escherichia coli T7-Notexin with the amino acid substitution D49KDepositorInsertNotexin
TagsT7 peptide tagExpressionBacterialMutationChanged Aspartic Acid 49 to LysinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP
Plasmid#58490PurposeExpresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltageDepositorInsertArchaerhodopsin-3 w/ D95H point mutation fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Aspartic Acid 95 to GlutaminePromoterUbiquitinAvailable SinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2m2-SNAP
Plasmid#208773PurposeTo express C2m2-SNAP in bacteriaDepositorInsertC2m2-SNAP (Mfge8 Mouse)
TagsSNAP-tagExpressionBacterialMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterT7 promoterAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMYH9-D1424H-FLAG
Plasmid#183513PurposeGateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.DepositorInsertMYH9 (MYH9 Human)
TagsFLAGExpressionMammalianMutationchanged Aspartic acid 1424 to HistidinePromoterCMVAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)
Plasmid#127392PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
TagsmEGFPExpressionMammalianMutationchanged Threonine 286 to Aspartic acid and Threon…PromoterpCAGAvailable SinceJuly 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
GANLS-DEVD-BNES
Plasmid#50835PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddGFP A and ddRFP B
TagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBG-MKK4-S255D/T259E (GST)
Plasmid#21566DepositorInsertmitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
TagsGSTExpressionMammalianMutationSerine 255 to Aspartic Acid, Threonine 259 to Gl…Available SinceJuly 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
TPC2(N257A)-mCherry
Plasmid#135186PurposePore-dead mutant of TPC2, tagged on C terminus with mCherry.DepositorInsertTPC2 (Tpcn2 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationMutation of asparagine257 to alanineAvailable SinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GANES-DEVD-BNLS
Plasmid#50842PurposeExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.DepositorInsertddGFP A and ddGFP B
TagsNES sequence LALKLAGLDIGS placed after ddGFP A an…ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEX-SP-YFP-STIM1(D76A)
Plasmid#18859DepositorInsertStromal interaction molecule 1 (STIM1 Human)
TagsYFPExpressionMammalianMutationD76A mutation - changed aspartate 76 to alanine. …Available SinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
RANLS-DEVD-BNES
Plasmid#50840PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddRFP A and ddRFP B
TagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianPromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-jYCaMP1
Plasmid#135423PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVExpressionMammalianPromoterhSynAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
NESRA-DEVD-BNLS
Plasmid#50849PurposeExpresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.DepositorInsertddRFP A and ddRFP B
TagsNES sequence LQKKLEELELDE placed after ddGFP A an…ExpressionMammalianPromoterCMVAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-HA-CAD S1859A
Plasmid#46239PurposeFLAG-HA-CAD with S1859 phosphorylation site mutated to alanineDepositorInsertcarbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
TagsFLAG and HAExpressionMammalianMutationSerine 1859 changed to Alanine (S1859A)PromotercmvAvailable SinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DEPTOR S286/287/291D
Plasmid#159161PurposeExpresses DEPTOR S286/287/291D mutant in mammalian cellsDepositorInsertDEPTOR (DEPTOR Human)
TagsHAExpressionMammalianMutationchanged serine 286,287 and 291 to aspartic acidPromoterCMVAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1mut
Plasmid#86690PurposeFor transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)
TagsHAMutationmutated region NHDC (asparagine 273 to cysteine 2…PromoterT3Available SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-SHP2DA
Plasmid#12286DepositorInsertSHP-2DA (PTPN11 Human)
TagsMycExpressionMammalianMutationAspartic Acid 425 mutated to AlanineAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2m2-mKate
Plasmid#208765PurposeTo express C2m2-mKate in bacteriaDepositorInsertC2m2-mKate (Mfge8 Mouse)
TagsmKateExpressionBacterialMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterT7 promoterAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAX-mScarlet-FBP17
Plasmid#178366PurposeExpresses FBP17 c-terminal labeled with mScarelt in mammalian cellsDepositorInsertFormin Binding Protein 17 (FNBP1 Human)
ExpressionMammalianMutation400 Glutamic Acid changed to Aspartic acid (403 A…PromoterChicken Beta ActinAvailable SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only