We narrowed to 2,968 results for: GAI

1. pORTMAGE311B

   Plasmid

   #120418

   Purpose

   Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

   Depositor

   Csaba Pál

   Article

   Szili et al bioRxiv (2018)

   Inserts

   MutL E32K
   Lambda Beta
   XylS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E32K mutation conferring dominant mutator phenoty…

   Promoter

   Pm

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

   Plasmid

   #207878

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

   Plasmid

   #192204

   Purpose

   Lenti-GL(GFP-Luciferase)

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-GL(GFP-Luciferase)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRIG-Akt1

   Plasmid

   #53583

   Purpose

   lentiviral expression of constitutively active human Akt1 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   cAkt1 (AKT1 Human)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   constitutively active (myristoylated version)

   Promoter

   CMV

   Available since

   Oct. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLY098-RetroEFS-BCMABBz-WPRE

   Plasmid

   #192199

   Purpose

   Retro-BCMACAR

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-BCMACAR (TNFRSF17 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLY093-EFS-BCMA-Blast-WPRE

   Plasmid

   #192194

   Purpose

   Lenti-BCMA-OE-Blast

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-BCMA-OE-Blast (TNFRSF17 Synthetic)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

   Plasmid

   #207880

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXD023 - pLKO-EF1a-Puro-P2A-FLuc-WPRE

   Plasmid

   #192203

   Purpose

   Lenti-PL(Puro-Luciferase)

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-PL(Puro-Luciferase)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-Bhlhe40-3xFLAG-IRES-BFP

   Plasmid

   #117263

   Purpose

   Retroviral overexpression of Bhlhe40 with a 3xFLAG

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Bhlhe40 (Bhlhe40 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMP71-HLA-B0702-His

    Plasmid

    #104850

    Purpose

    HLA-B0702 his tagged

    Depositor

    Sébastien Walchli

    Article

    Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

    Insert

    major histocompatibility complex, class I, B (HLA-B Human)
    

    Use

    Retroviral

    Tags

    his

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-XL4 ASXL1 (p.G646 Wfs *12) 3x FLAG

    Plasmid

    #74245

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.G646 Wfs *12 truncation

    Promoter

    CMV

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. MSCV-IRES-GFP ASXL1 (p.G646 Wfs *12) 3x FLAG

    Plasmid

    #81021

    Purpose

    Cancer-associated ASXL1 truncation mutant in retroviral vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.G646 Wfs *12 truncation

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

13. GFP-Bcl2-Cb5

    Plasmid

    #18000

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    ER targeted Bcl-2 (BCL2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Bcl-2's C terminal targeting sequence replac…

    Promoter

    CMV

    Available since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

14. GFP-Bcl2-Maob

    Plasmid

    #18001

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Mitochondria-targeted Bcl-2 (BCL2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Bcl-2's C terminal replaced with mitochondri…

    Promoter

    CMV

    Available since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pLY099-RetroEFS-BCMABBz-PRODH2-WPRE

    Plasmid

    #192200

    Purpose

    Retro-BCMACAR-PRODH2

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Retro-BCMACAR-PRODH2 (TNFRSF17 Synthetic)
    

    Use

    Retroviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV p2GM AMPK alpha2hp1 alpha1hp1

    Plasmid

    #89492

    Purpose

    shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

    Depositor

    Russell Jones

    Article

    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

    Insert

    2 hairpins against mouse/human AMPKalpha1 and alpha2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (The GFP-Puro is from a PGK promoter; the shRN…

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pENTR-HLA-A0201-His

    Plasmid

    #108213

    Purpose

    HLA-A0201 his tagged

    Depositor

    Sébastien Walchli

    Article

    Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

    Insert

    major histocompatibility complex, class I, B (HLA-A Human)
    

    Use

    Entry vector for gateway system

    Tags

    his

    Expression

    Mutation

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB1.HMBP.PrS.JARID2

    Plasmid

    #125166

    Purpose

    Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    JARID2 (JARID2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG

    Plasmid

    #81023

    Purpose

    Cancer-associated ASXL1 truncation mutant in retroviral vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains ASXL1 amino acids 1-479

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

20. PX458-sgTSC2

    Plasmid

    #128098

    Purpose

    CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    sgRNA against human TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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