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pRS414 Hsh155 N295D/D450G

Plasmid

#87287

Purpose

Encodes Hsh155 N295D D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

N295D & D450G

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/H331D

Plasmid

#87282

Purpose

Encodes Hsh155 R294L H331D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

R294L & H331D

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/H331D

Plasmid

#87285

Purpose

Encodes Hsh155 N295D H331D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

N295D & H331D

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/K335E

Plasmid

#87286

Purpose

Encodes Hsh155 N295D K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

N295D & K335E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/D450G

Plasmid

#87284

Purpose

Encodes Hsh155 R294L D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

R294L & D450G

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/K335E

Plasmid

#87283

Purpose

Encodes Hsh155 R294L K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

R294L & K335E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E224Q, GiVL-2)

Plasmid

#32035

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

E224Q and N195-M215 replaced by P697-D714 of GiDi…

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E147Q/D151N)

Plasmid

#32024

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

E147Q/D151N

Promoter

T7

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A

Plasmid

#18691

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A

Use

Tags

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
7861 pRS305-pUG72-HSC82out

Plasmid

#10899

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 URA3 (HSC82 Budding Yeast)

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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pRS423-GPD-wrmScarlet1-10-CYC1 terminator

Plasmid

#158584

Purpose

Expresses wrmScarlet1-10 in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF-DUET1-DHDDS/Sumo-NgBR

Plasmid

#203162

Purpose

Expression of human cis-prenyltransferase complex for purification

Depositor

William Sessa

Article

Edani et al Proc Natl Acad Sci U S A. 2020 Aug 25;11

Inserts

DHDDS (DHDDS Human)
NUS1 dehydrodolichyl diphosphate synthase subunit (NUS1 Human)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

truncation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

Plasmid

#198417

Purpose

Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

Depositor

Takumi Kamura

Article

Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

Insert

OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRS306-URA3-PHIS3-AqLumSynth –Sapphire

Plasmid

#116931

Purpose

Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

lumazine synthase

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

Plasmid

#193049

Purpose

Encodes G-418 resistance

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

prsp-27::NeoR::unc-54 3’ UTR

Use

Tags

Expression

Worm

Mutation

Promoter

prsp-27

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

Plasmid

#154987

Purpose

Lentiviral vector expressing Human ACE2 and TMPRSS2

Depositor

Sonja Best

Insert

ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Use

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS306-URA3-PHIS3-PfV-GS-Sapphire

Plasmid

#116932

Purpose

Yeast integrative vector for the exression of the 40nm GEM under the HIS3 promoter

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-6xHis mAb

Plasmid

#214952

Purpose

Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-6xHis mAb heavy chain
VNp-anti-6xHis mAb light chain

Use

Tags

VNp6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRS305-Leu2-PINO4-PfV-GS-Sapphire

Plasmid

#116930

Purpose

Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

INO4

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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