We narrowed to 17,638 results for: URE

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pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847

Plasmid

#91915

Purpose

Expresses Cg2Cas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

Cg2Cas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCVL TAL(Nd154C63) SFFV Xba-Sal linker

Plasmid

#50420

Purpose

pTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Lentiviral and TALEN

Expression

Mammalian

Promoter

SFFV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3B09

Plasmid

#70998

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Hrb87F (Hrb87F Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
Syntaxin1A(E53TAG)-mCherry

Plasmid

#241293

Purpose

Mammalian expression of syntaxin-1(E53TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E53TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E206TAG)-mCherry

Plasmid

#241295

Purpose

Mammalian expression of syntaxin-1(E206TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E206TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(K84TAG)-mCherry

Plasmid

#241297

Purpose

Mammalian expression of syntaxin-1(K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K84TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E49TAG)-mCherry

Plasmid

#241291

Purpose

Mammalian expression of syntaxin-1(E49TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E49TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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IMPT-11371

Plasmid

#236191

Purpose

Express GPR119 in insect cells

Depositor

Vadim Cherezov

Article

Kim et al Structure. 2024 Dec 5;32(12):2206-2219.e

Insert

GPR119 (GPR119 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS794

Plasmid

#238207

Purpose

Expresses native TaTasR TIGR locus, TasR has premature stop codon. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Mutation

A insertion at K85 codon

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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WDV

Plasmid

#226983

Purpose

Expresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experiments

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Insert

WDV (repA )

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTcI38-PRM-wasabi

Plasmid

#195554

Purpose

Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
TcI38

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T, M1V, L65S, K68R, F115L, D126G, D188G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-CI2F

Plasmid

#191254

Purpose

Expresses chymotrypsin inhibitor with an N-terminal MBP fusion protein

Depositor

Arthur Laganowsky

Article

Raab et al J Am Chem Soc. 2020 Oct 14;142(41):17372

Insert

Chymotrypsin Inhibitor 2

Tags

Maltose Binding Protein

Expression

Bacterial

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-HisTim12_CtoA

Plasmid

#170281

Purpose

Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

Insert

yeast Tim12 (TIM12 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag (on backbone)

Expression

Bacterial

Mutation

mutated two non-conserved Cys to Ala

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG

Plasmid

#158132

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG2

Plasmid

#158134

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the second serine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG3

Plasmid

#158136

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third alanine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG4

Plasmid

#158137

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSKB3-SaGGR-F219L (JBEI-7186)

Plasmid

#110151

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-F219L mutant gene

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4F04

Plasmid

#71030

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sin3A (Sin3A Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B10

Plasmid

#70840

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sce (Sce Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only