We narrowed to 3,254 results for: actin

1. plko-shmfn2-mfn2-Rescue

   Plasmid

   #160655

   Purpose

   "Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"

   Depositor

   Qing Deng

   Article

   Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

   Insert

   mfn2 (MFN2 Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR201-Eps8L2

   Plasmid

   #196526

   Purpose

   Entry vector for Gateway with Eps8L2

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2-FL (EPS8L2 Human)
   

   Expression

   Bacterial

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB80-hsKIF5B(1-560)Rigor-L-2xHA

   Plasmid

   #193718

   Purpose

   Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Human, Synthetic)
   

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

   Plasmid

   #174633

   Purpose

   Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Tags

   mCherry-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag Intersectin Short (human)

   Plasmid

   #47392

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   Intersectin short (ITSN1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCS2.0 Tag-RFP-T-UtrCH

   Plasmid

   #101279

   Purpose

   Life imaging pCS2.0 Tag-RFP-T_UtrCH

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   Utrophin (UTRN Human)
   

   Tags

   TagRFP-T

   Expression

   Mammalian

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-BASIC-ARHGAP29 PROMOTER

   Plasmid

   #104155

   Purpose

   luciferase reporter with ARHGAP29 promoter

   Depositor

   Marius Sudol

   Article

   Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

   Insert

   ARHGAP29 promoter (ARHGAP29 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   ARHGAP29

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMYs-MFN2-IRES-mitoRFP

   Plasmid

   #121997

   Purpose

   Expression of MFN2 and mitoRFP in mammalial cells

   Depositor

   Qing Deng

   Article

   Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

   Insert

   MFN2-IRES-mitoRFP (MFN2 Human)
   

   Use

   Retroviral

   Mutation

   silent Q386Q Q387Q V388V Y389Y C390C E391E E392E

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. ps-3HA-Beta2-PSCFP2-N

   Plasmid

   #114186

   Purpose

   studying clustering of Beta2 receptors by superresolution microscopy (PALM)

   Depositor

   Aleksandra Radenovic

   Article

   Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

   Insert

   Adrenergic receptor beta 2 (ADRB2 Human)
   

   Tags

   3HA and PS-CFP2

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid

    #131143

    Purpose

    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    mNeonGreen::3XFLAG::Crk (Crk Fly)
    

    Use

    Cre/Lox; Attb recombination site

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid

    #131137

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

    Plasmid

    #174634

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Tags

    mCherry-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

    Plasmid

    #193717

    Purpose

    Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Human, Synthetic)
    

    Tags

    2xmNeongreen-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-HypaCas9-mR2-ACTB_sgRNA

    Plasmid

    #183884

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTIGER_tdTomato::3xFLAG::dCrk

    Plasmid

    #131138

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and tdTomato

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid

    #193720

    Purpose

    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Human, Synthetic)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-CLCb (EED/QQN)

    Plasmid

    #47422

    Depositor

    Peter McPherson

    Article

    Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

    Insert

    CLCb EED/QQN (CLTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EED changed to QQN, in aa20-41 region

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Nup98-pDONR221

    Plasmid

    #124241

    Purpose

    Gateway entry clone of Drosophila Nup98-96 cDNA.

    Depositor

    Peter Vilmos

    Article

    Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

    Insert

    Drosophila Nup98-96 (Nup98-96 Fly)
    

    Expression

    Insect

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-Ctdnep1_Cter

    Plasmid

    #196522

    Purpose

    Entry vector for Gateway with Ctdnep1_Cter

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1-Cter (CTDNEP1 Human)
    

    Expression

    Bacterial

    Mutation

    No transmembrane domain

    Promoter

    CMV promoter; T7 promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR201-Ctdnep1

    Plasmid

    #196520

    Purpose

    Entry vector for Gateway with Ctdnep1

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1-N1 (CTDNEP1 Human)
    

    Expression

    Bacterial

    Promoter

    CMV promoter; T7 promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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