165,725 results

1. pCGN-MEF2B

   Plasmid

   #32959

   Purpose

   For mammalian expression of human MEF2B with an HA tag

   Depositor

   Ron Prywes

   Article

   Han et al Mol Cell Biol. 1995 Jun;15(6):2907-15.

   Insert

   MEF2B (MEF2B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-hACE2

   Plasmid

   #145033

   Purpose

   Express human ACE2 with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   human ACE2 (ACE2 Human)
   

   Tags

   C9

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLJM60 FLAG-RAB35 S22N

   Plasmid

   #107106

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP955

   Plasmid

   #98784

   Purpose

   Accessory Plasmid for PACE

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   proB-lysozyme-ggs-ENLYFQS-ggs-T7RNAP//T7pro-gIII-lux
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST/N1-hBIN1-GFP

   Plasmid

   #27305

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Insert

   bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-mEGFP-CaMKIIa

   Plasmid

   #127389

   Purpose

   Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Synapsin-pm-iATPSnFR1.0

   Plasmid

   #102555

   Purpose

   expresses membrane displayed ATP sensor in neurons

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Use

   AAV

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ10873

   Plasmid

   #183961

   Purpose

   U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   HyperdCas12a
   poly-crRNA array targeting Sox2-Klf4-Oct4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG and U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiGuide-hygro

   Plasmid

   #160090

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD649-HAsp-HLA-E-COMP5AP-AviTag-9xHis

    Plasmid

    #157396

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    HLA-E (HLA-E Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 HA E2F3A

    Plasmid

    #236437

    Purpose

    transient overexpression of E2F3A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3A (E2F3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO5.sgRNA.EFS.tRFP657

    Plasmid

    #57824

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAuroraA-GFP-AURKA

    Plasmid

    #99877

    Purpose

    Expression of GFP-AURKA in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    AURKA minimal

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas12j3 VPR

    Plasmid

    #188517

    Purpose

    Expresses FLAG tagged dCas12j3 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D413A

    Promoter

    EF1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IZ-ANXA7-mRuby3

    Plasmid

    #184893

    Purpose

    Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA7 (ANXA7 Human)
    

    Tags

    mRuby3

    Expression

    Mammalian

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVSV Venus VSV-G

    Plasmid

    #36399

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    VSV Genome
    

    Promoter

    VSV promoters

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRDB_053

    Plasmid

    #216083

    Purpose

    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    

    Use

    Destination

    Expression

    Insect

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

    Plasmid

    #232880

    Purpose

    Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    ADP-ribosylation factor-like protein 13B (ARL13B Human)
    

    Use

    Lentiviral

    Tags

    GFP and RGECO1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJRK246

    Plasmid

    #173741

    Purpose

    donor plasmid for SapTrap assembly

    Depositor

    Mike Boxem

    Article

    Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

    Insert

    wrt-2 promoter
    

    Use

    Other

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid

    #186310

    Purpose

    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg61-PDGFR
    eGFP
    

    Tags

    His, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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