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pAF9D92

Plasmid

#185846

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>TUP1>PABF1

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-FAK-HA

Plasmid

#35039

Depositor

Anna Huttenlocher

Article

Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

Insert

focal adhesion kinase (Ptk2 Mouse)

Tags

2x HA and mCherry

Expression

Mammalian

Mutation

Y42H; deletion of amino acids 1051 & 1052

Available Since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-DogTag

Plasmid

#105629

Purpose

Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SUMO-DogTag

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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Tailless VinculinTS

Plasmid

#26020

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

tailless vinculinTS

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TFEBS211A-MYC (#805)

Plasmid

#99957

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHIs and Myc

Expression

Mammalian

Mutation

S211A

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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PH-Akt(R25C)-GFP

Plasmid

#51466

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Mutation

R25C

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV PIG (Puro IRES GFP empty vector)

Plasmid

#21654

Purpose

Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV Puro

Plasmid

#68469

Purpose

Retroviral plasmid with multiple cloning site and puromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Retroviral

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGK415-rtetTAK8N, L131L

Plasmid

#165969

Purpose

Expresses rTetTA (K8N, L131L) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

tetR

Use

Synthetic Biology

Tags

three copies of VP16 (VP48)

Expression

Yeast

Promoter

pPGK1

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

Plasmid

#176271

Purpose

Expression of CasMINI-V3.1 in mammalian cells.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI-V3.1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Bxb1_tdTomato

Plasmid

#198041

Purpose

Bxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Insert

tdTomato

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE42

Plasmid

#90380

Purpose

GATA3 - Gata3 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

GATA3

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XHA-EGFP-PEX26

Plasmid

#139054

Purpose

HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XHA-EGFP-PEX26

Use

Lentiviral

Tags

HA

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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mNG2(1-10)_tdTomato

Plasmid

#184169

Purpose

Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(1-10)
tdTomato

Use

Lentiviral

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRS306:Met3p-mCherry-Tub1

Plasmid

#37554

Depositor

Wei-Lih Lee

Article

Markus et al Cytoskeleton (Hoboken). 2011 Mar;68(3):1

Insert

Met3p-mCherry-Tub1

Expression

Bacterial

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
pEZT-BM

Plasmid

#74099

Purpose

Multi-purpose rapid titer bacmam expression vector

Depositor

Ryan Hibbs

Article

Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Bacmam, baculovirus

Tags

EGFP following p10 promoter

Expression

Mammalian

Promoter

CMV, p10

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0

Plasmid

#153285

Purpose

Express the adenosine sensor GRAB_Ado1.0 in neurons

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Has Service

AAV1

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

hSyn

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgAP2S1 #2

Plasmid

#202757

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting AP2S1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgAP2S1 #1

Plasmid

#202756

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting AP2S1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pIKU801-CrPEPC1

Plasmid

#92342

Purpose

Expresses sgRNA for targeting CrPEPC1 gene, paromomycin resistance

Depositor

I-Son Ng

Article

Kao et al Bioresour Technol. 2017 May 4. pii: S096

Insert

sgRNA-CrPEPC1

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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