We narrowed to 49,092 results for: prot
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Plasmid#200371PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
ExpressionMammalianMutationNAAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF1
Plasmid#200372PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
ExpressionMammalianMutationNAAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF2
Plasmid#200373PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
ExpressionMammalianMutationNAAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF1
Plasmid#200378PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
ExpressionMammalianMutationNAAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF0
Plasmid#200377PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
ExpressionMammalianMutationNAAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF2
Plasmid#200379PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
ExpressionMammalianMutationNAAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
TagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAIDA1
Plasmid#79180PurposeA construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tagsDepositorInsertAdhesin Involved in Diffuse Adherence
TagsHis and MycExpressionBacterialPromoterlacUV5Available SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
TagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDG02583
Plasmid#104129PurposeOptimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)DepositorInsertNEDD8-specific protease 1 (NEDP1)
Tags14x Histidine tag, Maltose binding protein, and S…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEff
Plasmid#97423PurposeTransient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FMRP_P451S
Plasmid#198693PurposeExpression of human FMRP protein in E. coliDepositorInsertFMR1 (FMR1 Human)
TagsMBPExpressionBacterialMutationchanged proline 451 to serinePromoterT7Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP680-N1
Plasmid#136557PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)DepositorInsertmiRFP680
ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin
Plasmid#134858PurposeExpresses EGFP-tagged early endosome markerDepositorInsertRas-related protein 5A
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-GPI
Plasmid#65294Purposesynchronize trafficking of EGFP-GPI from the ER (RUSH system)DepositorInsertGPI anchor
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP670nano3
Plasmid#184663PurposeMonomeric near-infrared fluorescent protein miRFP670nano3 in bacterial plasmidDepositorInsertmiRFP670nano3
TagsHisExpressionBacterialPromoteraraCAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
VR124 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
Tags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1
Plasmid#175360PurposeZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 PlatformDepositorTypeEmpty backboneExpressionInsectPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only