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pNP1 eforRed toehold switch

Plasmid

#107353

Purpose

Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 1 + eforRed

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK564

Plasmid

#110613

Purpose

BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pWZL N-Myc-hPOT1_deltaOB

Plasmid

#166408

Purpose

express hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

POT1 ΔOB (POT1 Human)

Tags

myc

Expression

Mammalian

Mutation

ΔOB

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pUb dRxCas13d + RxCas13d guide RNA

Plasmid

#176304

Purpose

Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

RxCas13d

Tags

HA tag

Expression

Insect

Mutation

R239A, H244A, R858A, H863A

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1d

Plasmid

#134906

Purpose

Expression of rat Myo 1d (Myr 4) in mammalian cells.

Depositor

Martin Bähler

Article

Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

Insert

Myo 1d (rat) with 3' UTR (Myo1d Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFAB808

Plasmid

#47853

Purpose

BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rrnB T1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO8

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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THI VP64 pALD4 T-

Plasmid

#161481

Purpose

Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Non targeting control NTC)

Expression

Yeast

Promoter

pALD4, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-GW182delta1

Plasmid

#11592

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

GW182 (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contains only aa1-498; SEE DEPOSITOR NOTES BELOW

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pDGE657

Plasmid

#153255

Purpose

M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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NFAST(low)-mApple-6xHp

Plasmid

#214412

Purpose

Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Tags

mApple-6xHp

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pISceI Dusp6:memGFP

Plasmid

#32668

Purpose

Expresses membrane localized EGFP under the Dusp6 promoter

Depositor

Michael Tsang

Article

Wang et al Development. 2011 Jan;138(1):45-54. Epub

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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IFI16-(192-393)-HinA (pcDNA3-FLAG)

Plasmid

#35062

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

FLAG and His

Expression

Mammalian

Mutation

Contains HinA domain: aa 192-393

Promoter

CMV

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pMpGWB331

Plasmid

#68659

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Emerald Luc fused with PEST sequence [ELuc(PEST)]

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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IFI16-(515-710)-HinB (pcDNA3-FLAG)

Plasmid

#35063

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(515-710)-HinB (IFI16 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains HinB domain: aa 515-710

Promoter

CMV

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pMIG II SIN-SV40-mCherry-SHP2

Plasmid

#180800

Purpose

Expression of mouse SHP2 fused to N-terminal mCherry

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SHP2 (Ptpn11 Mouse)

Use

Retroviral

Promoter

SV40

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKC158

Plasmid

#134242

Purpose

CENP-A Halo expression construct

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP-A-Halo tagging construct (CENPA Human)

Use

Human targeting

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB316

Plasmid

#68644

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTSSX-STC

Plasmid

#115668

Purpose

E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

Depositor

David Kramer

Article

Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

Insert

small tetraheme cytochrome c

Tags

Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

Expression

Bacterial

Promoter

tac

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pOBCol3.6-GFPtpz

Plasmid

#110211

Purpose

Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPtopaz

Tags

None

Expression

Mammalian

Mutation

None

Promoter

3.6 kb rat type 1 collagen + first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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RBM4_2xRRM-ZNF_pGEX

Plasmid

#135154

Purpose

Recombinant expression of RBM4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM4 (RBM4 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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