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Showing: 10641 - 10660 of 18247 results

  2. pKF-D2irTNP2AG-T2APuroR-5kwh

    Plasmid
    #128255
    Purpose
    Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.
    Depositor
    Gabor Balazsi
    Publication
    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hTetR::P2A::EGFP::T2A::PuroR
    Use
    Synthetic Biology; Flp-in expression vector
    Expression
    Mammalian
    Promoter
    CMV-D2ir promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    128255-plasmid-map-sequence-id-248943

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  3. pFGL1079

    Plasmid
    #116897
    Purpose
    CenpC:eGFP (centromere marker)
    Depositor
    Naweed Naqvi
    Publication
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cenp C (partial)
    eGFP
    CenpC downstream region
    Use
    Fungal expression (in magnaporthe oryzae)
    Mutation
    truncated, no stop codon and without start codon
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    116897-plasmid-map-sequence-id-232134

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  4. pMTG8-Amp-FRT

    Plasmid
    #128277
    Purpose
    TIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFP
    Depositor
    Gabor Balazsi
    Publication
    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    TetR-LOV-TIP
    EGFP
    KRAS(G12V)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128277-plasmid-map-sequence-id-251922

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  5. pEG302 22aa SunTag NtDRMcd SUP

    Plasmid
    #115489
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    115489-plasmid-map-sequence-id-242029

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  6. pEG302 5aa SunTag VP64 g4+g17 (FWA)

    Plasmid
    #119672
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    119672-plasmid-map-sequence-id-242023

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  7. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113687
    Purpose
    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113687-plasmid-map-sequence-id-231825

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  9. ACTIN-Cxcl5-PGK-Cre

    Plasmid
    #110278
    Purpose
    Bicistronic lentivirus expressing Cxcl5 and Cre
    Depositor
    Trudy Oliver
    Publication
    Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.
    Inserts
    chemokine (C-X-C motif) ligand 5 (Cxcl5 Mouse)
    cyclization recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    No fusion genes
    Expression
    Mammalian
    Mutation
    Wild type
    Promoter
    B-actin and Pgk
    Available since
    Oct. 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. pFUSEss-CHIg-mG3_CH3-1

    Plasmid
    #105859
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH3.
    Depositor
    Joanna Bereta
    Publication
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG3 with CH3 derived…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    105859-plasmid-map-sequence-id-206031

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  11. pFUSEss-CHIg-mG3_CH2-1

    Plasmid
    #105857
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH2.
    Depositor
    Joanna Bereta
    Publication
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG3 with CH2 derived…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    105857-plasmid-map-sequence-id-206029

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  12. pFUSEss-CHIg-mG3_CH1h-1

    Plasmid
    #105856
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH1 and hinge.
    Depositor
    Joanna Bereta
    Publication
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1, IgG3 (Ighg1, Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG3 with CH1 and hin…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    105856-plasmid-map-sequence-id-206028

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  13. AAEL007584-Cas9

    Plasmid
    #100706
    Purpose
    The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Publication
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL007584-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Promoter
    AAEL007584 and Opie2
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100706-plasmid-map-sequence-id-193543

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  15. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid
    #82469
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Publication
    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d
    Insert
    mRNA capping enzyme (Rngtt Mouse)
    Tags
    Flag and Myc
    Expression
    Mammalian
    Mutation
    changed Lysine 294 to Alanine
    Promoter
    CMV
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    82469-plasmid-map-sequence-id-163073

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  17. pETDuet-msGC-NT13

    Plasmid
    #75087
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Publication
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 4…
    Available since
    May 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    75087-plasmid-map-sequence-id-148282

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  18. pSMART-2,3-BDO1

    Plasmid
    #65816
    Purpose
    NADH Dependent 2,3-BDO Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    budA: α-acetolactate decarboxylase
    budB: acetolactate synthase
    budC: acetoin reductase
    Expression
    Bacterial
    Promoter
    waaHp from E. coli as part of operon with budA an…
    Available since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    65816-plasmid-map-sequence-id-111948

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  20. pLSLZ

    Plasmid
    #51495
    Purpose
    pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    Zif268:FokI
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    SV40 NLS
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51495-plasmid-map-sequence-id-78592

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Showing: 10641 - 10660 of 18247 results