We narrowed to 92,138 results for: lias

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pcDNA3.1+_FH-AGO2-WT

Plasmid

#92006

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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hyBE4max

Plasmid

#157942

Purpose

Mammalian expression of hyBE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

hyBE4max

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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eTd-CBEm

Plasmid

#196602

Purpose

Mammalian expression of eTd-CBEm

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA

Plasmid

#108748

Purpose

Expresses aa28 to C-terminus of Flp recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa28 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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MSP2135

Plasmid

#72248

Purpose

Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, and D1135E

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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ITPKA-mTurquoise2

Plasmid

#137811

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA (ITPKA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pST1374-scFv-APOBEC-UGI-GB1

Plasmid

#113029

Purpose

Expresses scFv-rAPOBEC1-UGI in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

scFv-rAPOBEC1-UGI-GB1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His-mPer1

Plasmid

#219421

Purpose

Expresses 3873 bp of mPer1 coding sequence in E. coli.

Depositor

Aziz Sancar

Article

Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mPer1 (Per1 Mouse)

Tags

His and V5

Expression

Bacterial and Mammalian

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-tagRFP-BleoR

Plasmid

#192508

Purpose

Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

Depositor

Gerald Schwank

Article

Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

PE2-P2A-tagRFP-P2A-BleoR

Tags

BleoR, SV40 bpNLS, and tagRFP

Expression

Mammalian

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-eBE

Plasmid

#107688

Purpose

Expresses dLbCpf1-eBE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-eBE

Expression

Mammalian

Mutation

dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

Plasmid

#163543

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_Anc689-NG-nCas9

Plasmid

#163527

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

Anc689-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3G-BE3

Plasmid

#113415

Purpose

Expresses hA3G-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3G-BE3 (APOBEC3G Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti PA-mCit

Plasmid

#113451

Purpose

Lentiviral ProteinA-mCitrine expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Use

Lentiviral

Expression

Mammalian

Promoter

hUBbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLCKO_Luciferase_sgRNA

Plasmid

#74190

Purpose

lentiviral vector expressing sgRNA targeting Luciferase

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

Luciferase sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

Plasmid

#167313

Purpose

Template pDNA to prepare DD-CaVT mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

DD-CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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eTd-CBEa

Plasmid

#196603

Purpose

Mammalian expression of eTd-CBEa

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-MED18

Plasmid

#15419

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED18 (MED18 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
OptoProfilin

Plasmid

#208286

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.WT

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin.WT (Cry2, PRF1 Mouse, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

Profilin WT (Mus musculus)

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-6-C, 16-C

Plasmid

#124681

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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