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pNX3s2-HA3

Plasmid

#78396

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
zeoCV

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a-FL3

Plasmid

#78358

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
kanMX6

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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EZH2-TALEN_Left_No_FLAG

Plasmid

#79899

Purpose

Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Left_No_FLAG

Use

TALEN

Expression

Mammalian

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBTR(SMCytc)

Plasmid

#72330

Purpose

Expresses spider monkey cytochrome c along with the heme lyase (CYC3) from yeast for heme insertion

Depositor

Bruce Bowler

Insert

spider monkey cytochrome c gene

Expression

Bacterial

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only
pYTK093

Plasmid

#65200

Purpose

Encodes LEU2 5' Homology as a Type 8b part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

LEU2 5' Homology

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK087

Plasmid

#65194

Purpose

Encodes LEU2 3' Homology as a Type 7 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

LEU2 3' Homology

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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LIIbeta R 1-2

Plasmid

#54357

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pISH-H2-M9

Plasmid

#15868

Depositor

Peter Mombaerts

Article

Ishii et al Curr Biol. 2003 Mar 4. 13(5):394-400.

Insert

H2-M9 (H2-M9 Mouse)

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only
pDE43-MCKq26

Plasmid

#27336

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

qtag26

Use

Gateway Cloning

Mutation

N/A

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pAL10

Plasmid

#236006

Purpose

Backbone for assembling gRNA arrays with LacZ gene for blue white screening

Depositor

Carl de Boer

Article

Salaudeen et al Synth Biol (Oxf). 2025 Dec 18;11(1):ysaf

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mScarlet-I-QtSig

Plasmid

#236775

Purpose

Expresses the red fluorescent protein mScarlet-I fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

HA_DD-N_mScarlet-I_QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK191

Plasmid

#226658

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, GentR, contains HIP1 site

Depositor

David Nielsen

Article

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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p140Tol2-Olactb:SABER

Plasmid

#226829

Purpose

Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-SABER
mCherry-t2A-CreERT2

Use

Zebrafish expression

Promoter

Olactb and hsp70

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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HisMS2_VLP_FluB

Plasmid

#175956

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza B NSP 1 protein [B/Colorado/06/2017]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Insert

NEP gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGEARBOCS-v0-Gfap- TagIN-HA

Plasmid

#196491

Purpose

To tag endogenous mouse GFAP with HA

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Inserts

HA
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJM195A

Plasmid

#218125

Purpose

Hybrid circuit displaying hsCRBN-CTD bait and driving expression of gIII

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN_CTD
M13 pIII

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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mOrange in pBSDONR P4r-P2

Plasmid

#121529

Purpose

For making C-terminal mOrange fusions using multisite Gateway cloning

Depositor

Roger Innes

Insert

mOrange

Expression

Bacterial

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SH2

Plasmid

#209704

Purpose

Mammalian expression of SH2

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

SH2

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTG131

Plasmid

#198640

Purpose

Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

Cas9

Expression

Bacterial

Promoter

pPhlF

Available Since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEY21

Plasmid

#191027

Purpose

ggr-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ggr-3

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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