We narrowed to 4,352 results for: hal.1

1. pSALECTNK-TEM1(S70A,D179G)/csTEM1

   Plasmid

   #81163

   Purpose

   pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

   Insert

   TEM-1 beta-lactamase
   

   Use

   Tags

   Codon swapped TEM-1 from H26 to W290 and His6x

   Expression

   Bacterial

   Mutation

   Deleted the sec transport tag. Plasmid encodes H2…

   Promoter

   lac promoter

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEY88

   Plasmid

   #191081

   Purpose

   ggr-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   ggr-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MBP_Lims (MBP_LIM1234)

   Plasmid

   #182042

   Purpose

   For expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.

   Depositor

   Vesa Hytönen

   Article

   Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

   Insert

   LIM1234 (PXN Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   LIM domains 1-4 only

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PARP1_D770A-mCherry

   Plasmid

   #192260

   Purpose

   Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

   Depositor

   Michael Cohen

   Article

   Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

   Insert

   PARP1_D770A (PARP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D770A

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PARP1_R878A-mCherry

   Plasmid

   #192261

   Purpose

   Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

   Depositor

   Michael Cohen

   Article

   Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

   Insert

   PARP1_R878A (PARP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R878A

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. t1-400

   Plasmid

   #166577

   Purpose

   For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

   Depositor

   Vesa Hytönen

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

   Insert

   Tln1 Head (1-400) (TLN1 Human)
   

   Use

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   residues 1-400 only

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH4040

   Plasmid

   #229803

   Purpose

   Pgpc-1 wCherry unc-54 3' UTR C.elegans neuron and other structures expression of RFP

   Depositor

   Mei Zhen

   Article

   Lin et al Sci Adv. 2023 Mar;9(9):eade1249. doi: 10

   Insert

   Pgpc-1 wCherry unc-54 3' UTR
   

   Use

   Tags

   RFP

   Expression

   Worm

   Mutation

   Promoter

   Pgpc-1

   Available since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. actc1b-2PH-PLCd-mKate2

   Plasmid

   #109500

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-2PH-PLCd-mKate2
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRY2low-tdTomato-Raf1

   Plasmid

   #104176

   Purpose

   Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato and Raf1

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2low-tdTomato-Raf1 (CRY2, RAF1 Mustard Weed, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CRY2 aa 1-491 truncation, R489E A491D

   Promoter

   CMV

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRY2low-mCherry-Raf1

    Plasmid

    #104066

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-mCherry-Raf1 (CRY2, RAF1 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEY141

    Plasmid

    #223488

    Purpose

    trp-4(intron 1) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    trp-4(intron 1) (trp-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMVTNT-T7-KPNA1

    Plasmid

    #26677

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 1 (KPNA1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    KPNA1 sequence contains a reported SNP at S73N

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. GAG-PEmax V7

    Plasmid

    #232921

    Purpose

    Optimized construct for the expression of PEmax fused to GAG from fMLV. This plasmid is involved in the production of NanoScribes

    Depositor

    Philippe Mangeot

    Article

    Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1

    Insert

    GAG FMLV fused to PEmax
    

    Use

    CRISPR

    Tags

    Nuclear export signals (x3)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac_His-ZZ-TEV-Lis1

    Plasmid

    #132539

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

    Depositor

    Andrew Carter

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker_TEV, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1BR9-AtWUSSTOP

    Plasmid

    #202053

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized (WUS Mustard Weed)
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7gRNA-smyhc1_977

    Plasmid

    #140867

    Purpose

    sgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    zebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Zebrafish, Synthetic)
    

    Use

    In vitro transcription of sgrnas

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZA12

    Plasmid

    #158490

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1 (ZAR1 Mustard Weed)
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    L17E, D489V, no STOP codon

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166010

    Purpose

    mammalian expression of SARS-CoV-2 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) (S )
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEY43

    Plasmid

    #191047

    Purpose

    eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    eat-4(prom6-1) (eat-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3 Flag Jip1 JBD

    Plasmid

    #15751

    Purpose

    binds to Jnk

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jip1 (Jnk binding domain) (Mapk8ip1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted 1-126 and 282-660

    Promoter

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only