Addgene

We narrowed to 2,103 results for: MP

Showing: 1591 - 1620 of 2103 results

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pCF160_VSV-G

Plasmid

#225963

Purpose

CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

Insert

CMV-Intron-VSVG (env protein)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-10XHis-GST-HRV-dL5

Plasmid

#73214

Purpose

Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

Insert

EGFR (EGFR Human, Schistosoma japonicum)

Use

Tags

10XHis-GST-HRV

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-RAB10 T23N

Plasmid

#130885

Purpose

Expresses human RAB10 T23N mutant with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

T23N

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_3xFLAG_V5_ eGFP_V5

Plasmid

#160111

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Lentiviral

Tags

3xFLAG, V5

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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GPS-MYC

Plasmid

#160130

Purpose

A reporter that monitors MYC protein expression.

Depositor

Channing Der

Article

Blake et al Sci Signal. 2019 Jul 16;12(590). pii: 12

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3

Plasmid

#136959

Purpose

Reporter vector encoding firefly luciferase and TurboRFP fluorescent protein

Depositor

Jakob Reiser

Article

Gills et al Oncotarget. 2018 Aug 24;9(66):32718-3272

Insert

Firefly luciferase and TurboRFP

Use

Lentiviral

Tags

V5 tag

Expression

Mutation

Promoter

CMV

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP12-Fibrillarin-7

Plasmid

#56252

Purpose

Localization: Nuclear - Nucleolus, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Fibrillarin (FBL Human)

Use

Tags

mIFP12

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-TRE3G-FNLS-PGK-Puro

Plasmid

#110847

Purpose

Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A and NLS sequence at the N-terminus

Promoter

TRE3G

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-KIAA0947

Plasmid

#49248

Purpose

Mammalian expression of flag-tagged human KIAA0947

Depositor

Ronald Conaway, Joan Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

KIAA0947 (ICE1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pCF140_Gag-SpyCas9-NLS

Plasmid

#225959

Purpose

CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

Insert

CMV-Intron-Gag-SpyCas9-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST53-LRRK2-R1441C

Plasmid

#25046

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Arginine 1441 mutated to Cysteine

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pCF153_Gag-Pol

Plasmid

#225961

Purpose

CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

Insert

CMV-Intron-GagPol (FMLV)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_1_001

Plasmid

#167347

Purpose

ddPCR gating control for droplets containing all 5 targets (2 in pol)

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_9_003

Plasmid

#167349

Purpose

ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_6_002

Plasmid

#167348

Purpose

ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_29_006

Plasmid

#167352

Purpose

ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_14_004

Plasmid

#167350

Purpose

ddPCR gating control for droplets containing the env target

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_32_007

Plasmid

#167353

Purpose

ddPCR gating control for droplets containing 5'pol or 3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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HA-EglN3 H196A-pcDNA3

Plasmid

#22717

Depositor

William Kaelin

Article

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 3 (EGLN3 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

mutant cDNA: point mutation at codon 196 was ins…

Promoter

Available since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-P2A-GFP-PGK-Puro

Plasmid

#110866

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med26

Plasmid

#49250

Purpose

Mammalian expression of flag-tagged Med26

Depositor

Ronald Conaway, Joan Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-2X-P2A-Puro

Plasmid

#110840

Purpose

Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-2X

Use

Lentiviral

Tags

Expression

Mutation

D10A and 2 tandem NLS sequences in the XTEN linker

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCIRCNpu

Plasmid

#74226

Purpose

Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

Depositor

Roland Eils, Barbara Di Ventura

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

Npu DnaE C-intein and Npu DnaE N-intein

Expression

Bacterial

Mutation

Promoter

T7-lac

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_empty

Plasmid

#160093

Purpose

Control lentiviral vector for mammalian gene expression

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP-H2B-C-10

Plasmid

#56224

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Use

Tags

mIFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

Plasmid

#202760

Purpose

Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

huAsCpf1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH

Plasmid

#105981

Purpose

This AAV plasmid constitutively expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

Depositor

Marcel Bruchez

Article

Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

Insert

hSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH

Use

AAV

Tags

FAPdL5-POST, T2A-dTomato, and myc

Expression

Mutation

Promoter

human synapsin 1

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCLHCX-mPM20D1-flag

Plasmid

#84566

Purpose

mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

Depositor

Bruce Spiegelman

Article

Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

Insert

peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

Retroviral

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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alpha actinin delta ABD-GFP

Plasmid

#66935

Purpose

Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

Depositor

Michael Sheetz, Pere Roca-Cusachs

Article

Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Alpha-actinin 1 (ACTN1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

actin binding domain (amino acids 30–253) is dele…

Promoter

CMV

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only