Addgene

We narrowed to 4,343 results for: Ado;

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SB-SRT-Puro

Plasmid

#154888

Purpose

Sleeping Beauty self-reporting transposon with puromycin reporter

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

Sleeping beauty SRT

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_SPIB_P2A_Hygro_Barcode

Plasmid

#120483

Purpose

Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIB (SPIB Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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PB-SRT-Puro

Plasmid

#154884

Purpose

piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

piggyBac SRT

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-E6AP Hect

Plasmid

#165102

Purpose

bacterial expression of a GST fusion of Human E6AP Hect domain

Depositor

Arthur Haas

Article

Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

Insert

E6AP Hect (UBE3A Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

E6AP Hect domain Amino acids 495-738 (5'QLNP…

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBOB-CARMIL2 BH domain-tdTomato

Plasmid

#118737

Purpose

To express CARMIL2 tagged BH with tdTomato on C-terminus

Depositor

John Cooper

Article

Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91.

Insert

CARMIL2 BH domain (CARMIL2 Human)

Use

Lentiviral

Tags

tdTomato

Expression

Mutation

Promoter

CMV

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMT-Map7-1333-end

Plasmid

#46070

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-1333-end (Map7 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-mRuby2

Plasmid

#99117

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Plasmid

#125225

Purpose

Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_TFE3_2

Plasmid

#86326

Purpose

Encodes gRNA for 3' target of human TFE3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TFE3 (TFE3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJET1.2-U6

Plasmid

#173157

Purpose

Single guide RNA cassette under U6 promoter

Depositor

Piotr Ziolkowski

Article

Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

Insert

sgRNA cassette under U6 promoter

Use

Tags

Expression

Plant

Mutation

Promoter

AtU6-26

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFETCh_CIC_iso1

Plasmid

#135728

Purpose

Donor vector for 3' FLAG tag of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 Homology arms (CIC Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-lxlplap

Plasmid

#45581

Depositor

Nirao Shah

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Inserts

Green fluorescent protein
Placental alkaline phosphatase (ALPP Human)

Use

Lentiviral

Tags

Histone 2B

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

Plasmid

#125230

Purpose

For generating SPARK NanoLuc-oβ2AR transmembrane component lentivirus

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

Use

Lentiviral

Tags

HA,Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-A2aTr316-H10

Plasmid

#121683

Purpose

Bacterial expression of adenosine A2a receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

Insert

adenosine A2a receptor (ADORA2A Human)

Use

Tags

His10

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2mFoxd3

Plasmid

#37267

Depositor

Patricia Labosky

Article

Steiner et al Development. 2006 Dec;133(24):4827-38. E

Insert

Foxd3 (Foxd3 Mouse)

Use

Overexpression

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
PX458_CIC_iso1_2

Plasmid

#135744

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_CIC_iso1_1

Plasmid

#135743

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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LifeAct-TagBFP-DiB-RM

Plasmid

#168782

Purpose

Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

LifeAct-TagBFP-DiB-RM

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-tLuc

Plasmid

#107552

Purpose

tLuc in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Luciferase

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_SPIC_P2A_Hygro_Barcode

Plasmid

#120484

Purpose

Barcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIC (SPIC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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