We narrowed to 15,384 results for: pET

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NC11 pCDNA3.1 VENUS WTX K292N

Plasmid

#36966

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX K292N (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

K292N

Promoter

CMV

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
NC8 pCDNA3.1 VENUS WTX S286D

Plasmid

#36963

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX S286D (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

S286D

Promoter

CMV

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
NC7 pCDNA3.1 VENUS WTX S286A

Plasmid

#36962

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX S286A (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

S286A

Promoter

CMV

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
NC10 pCDNA3.1 VENUS WTX T289A

Plasmid

#36965

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX T289A (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

T289A

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
NC9 pCDNA3.1 VENUS WTX S286E

Plasmid

#36964

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX S286E (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

S286E

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3

Plasmid

#136364

Purpose

Expresses epitope-tagged human TET3 WT in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Tags

2xStrep-tagII, FLAG, and HA

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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HDM_SARS2_Spike_del21_D614G

Plasmid

#158762

Purpose

Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614

Depositor

Jesse Bloom

Article

Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

Insert

SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

deleted amino acids 1257-1278, changed aspartic a…

Promoter

CMV

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.5

Plasmid

#155032

Purpose

Fluorescent reporter for ATF4 translation

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP

Plasmid

#204821

Purpose

Lentiviral vector for human SCGB3A2 promoter-driven expression of EGFP

Depositor

Emma Rawlins

Article

He et al Cell. 2022 Dec 8;185(25):4841-4860.e25.

Insert

An upstream region including human SCGB3A2 promoter

Use

Lentiviral

Expression

Mammalian

Promoter

SCGB3A2 promoter

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgCrebbp#2/Cre

Plasmid

#193210

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCrebbp#2 (Crebbp Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::lacZ-H11

Plasmid

#139099

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Hsp68

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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PB09-TRE-beta-globin-miR-124-EF1a-GFP

Plasmid

#117318

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Transposon-mediated integration

Tags

GFP

Expression

Mammalian

Promoter

Tet-inducible promoter

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

Plasmid

#117319

Purpose

Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-CTRL

Use

Transposon-mediated integration

Tags

GFP

Expression

Mammalian

Mutation

miR-124 deleted

Promoter

Tet-inducible promoter

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.12

Plasmid

#155033

Purpose

Fluorescent reporter for ATF4 translation, Negative Control

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 start codon mutated

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.14

Plasmid

#155034

Purpose

Fluorescent reporter for ATF4 translation, Positive Control

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 and uORF2 start codons mutated

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Mito-Pericam

Plasmid

#87381

Purpose

Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.

Depositor

Björn Stork

Article

Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

Insert

Mito-Pericam [ratiometric]

Use

Retroviral

Expression

Mammalian

Promoter

Retroviral 5' LTR promoter

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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CHFR-PBZ-eGFP

Plasmid

#211581

Purpose

Expressing CHFR PBZ domain fused to eGFP

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

CHFR PBZ domain (CHFR Human)

Tags

eGFP

Expression

Mammalian

Mutation

PBZ domain of the protein

Promoter

CMV

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

Plasmid

#236246

Purpose

Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

U6 and human Synapsin1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMG023 MBP-Axin 384-518-His (miniAxin)

Plasmid

#154012

Purpose

Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Tags

His6 and MBP

Expression

Bacterial

Mutation

381-518 fragment

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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