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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,530 results for: scr

Showing: 10901 - 10920 of 22530 results

  5. T7-TetO-GFP_ColE1

    Plasmid
    #173193
    Purpose
    Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    T7-TetO
    Use
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    173193-plasmid-map-sequence-id-339027

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  12. AAVS1-TRE3G-NODALvar-matMYC

    Plasmid
    #115641
    Purpose
    For targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycycline
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL splice variant (NODAL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115641-plasmid-map-sequence-id-232862

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  13. NODAL-MYC-DYK-N72A-N199A

    Plasmid
    #115252
    Purpose
    For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N72A, N199A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115252-plasmid-map-sequence-id-222234

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  14. NODAL-MYC-DYK-C312A

    Plasmid
    #115253
    Purpose
    For mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    C312A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115253-plasmid-map-sequence-id-222237

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  15. NODALvar-MYC-DYK-C302S

    Plasmid
    #115254
    Purpose
    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    C302S
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115254-plasmid-map-sequence-id-222242

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  16. NODALvar-MYC-DYK-C302A

    Plasmid
    #115255
    Purpose
    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    C302A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115255-plasmid-map-sequence-id-222245

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  17. NODALvar-MYC-DYK-N328A

    Plasmid
    #115246
    Purpose
    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL splice variant (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N328A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115246-plasmid-map-sequence-id-222166

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  18. pKDR-CBh.22

    Plasmid
    #183566
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 22
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183566-plasmid-map-sequence-id-370310

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  19. pKDR-EF1a.13

    Plasmid
    #183576
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 13
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183576-plasmid-map-sequence-id-370309

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Showing: 10901 - 10920 of 22530 results