We narrowed to 18,503 results for: Met

1. C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

   Plasmid

   #162933

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-maltose binding protein fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRWJB006

   Plasmid

   #160113

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Promoter

   35S

   Available Since

   Feb. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRWJB004

   Plasmid

   #160112

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Promoter

   UBQ10

   Available Since

   Feb. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMKO.1-shSpc25

   Plasmid

   #160960

   Purpose

   Spc25 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Spc25 shRNA (Spc25 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKH-1699

   Plasmid

   #124227

   Purpose

   Mammalian SpCas9-HF1 expression

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDUAL-Pnmt1-Ost4-GFP

   Plasmid

   #154368

   Purpose

   Yeast expression of Ost4

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   Ost4 (ost4 Fission Yeast)
   

   Expression

   Yeast

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-LAP1-mCherry

   Plasmid

   #159525

   Purpose

   Expresses mCherry from LAP1 promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

   Insert

   mCherry
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   LAP1

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC_h7SK M15 (UUA)

   Plasmid

   #154785

   Purpose

   plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   Mma PylT
   

   Expression

   Mammalian

   Mutation

   UUA anticodon and G10A, G14U, G16U, C20U, C52A

   Promoter

   h7SK

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAL-C2X dAmp/Kana+-2MT

   Plasmid

   #154353

   Purpose

   Bacterial expression of 2MT

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   2MT
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDUAL-P41nmt1-Sad1-GFP

    Plasmid

    #154366

    Purpose

    Yeast expression of Sad1

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Sad1 (sad1 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDUAL-P41nmt1-Nup124-GFP

    Plasmid

    #154364

    Purpose

    Yeast expression of Nup124

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Nup124 (nup124 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDUAL-Pnmt1-Ost4-GFP-MTalpha

    Plasmid

    #154370

    Purpose

    Yeast expression of Ost4

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Ost4 (ost4 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDUAL-P41nmt1-Sad1-GFP-MTn

    Plasmid

    #154367

    Purpose

    Yeast expression of Sad1

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Sad1 (sad1 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Antares-SEPLuc IRESv24

    Plasmid

    #141087

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEPLuc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBMN-AS-FOXA1

    Plasmid

    #139304

    Purpose

    Knocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1

    Depositor

    Claes Wadelius

    Article

    Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

    Insert

    shRNA and amiRNA against FOXA1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY71-tet.I.-1

    Plasmid

    #133539

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY71-tet.I.25

    Plasmid

    #133538

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pY71-tet.I.20

    Plasmid

    #133537

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY71-tet.I.14

    Plasmid

    #133535

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pY71-tet.I.13

    Plasmid

    #133534

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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