We narrowed to 12,750 results for: CAR

1. pEGFP-N1 alpha-actinin 1

   Plasmid

   #11908

   Depositor

   Carol Otey

   Article

   Edlund et al Cell Motil Cytoskeleton. 2001 Mar . 48(3

   Insert

   alpha-actinin 1 (ACTN1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pComb3XLambda

   Plasmid

   #63892

   Purpose

   Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   human antibody lambda light chain (IGLL5 Human)
   

   Use

   Phage display

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Keratin 8 (pcDNA3)

   Plasmid

   #18063

   Depositor

   M Bishr Omary

   Article

   Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

   Insert

   Keratin 8 (KRT8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-GST-AGO2

   Plasmid

   #24317

   Depositor

   Carl Novina

   Article

   Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

   Insert

   human AGO2 (AGO2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. 35S-GWY-GAL4BD/pAM

   Plasmid

   #201231

   Purpose

   Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

   Depositor

   Michael Ayliffe

   Article

   Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

   Insert

   35S-GWY-GAL4BD
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTF-lacZ

   Plasmid

   #153036

   Purpose

   For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-GFP

   Plasmid

   #137763

   Purpose

   Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GFP
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAD-sfGFP(Sp)-Pman

   Plasmid

   #133833

   Purpose

   Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(Sp)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pman

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG:: mCherry-2a-hM4Dnrxn

    Plasmid

    #52523

    Purpose

    Co-expresses hM4Dnrxn and a red fluorescent label

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    mCherry-2a-hM4Dnrxn
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAg

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-CD68 promoter/enhancer

    Plasmid

    #34837

    Depositor

    Peter Murray

    Article

    Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

    Insert

    CD68 promoter/enhancer -680/+140 (CD68 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEos2-Dectin1A-C-10

    Plasmid

    #57367

    Purpose

    Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Dectin1A (CLEC7A Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. Lenti-PEAR-mCherry

    Plasmid

    #228892

    Purpose

    All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Insert

    EGFP-IRES-mScarlet
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWM_12x601_15bpLinker

    Plasmid

    #157786

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_15bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Halo-VSVG-RUSH

    Plasmid

    #166907

    Purpose

    RUSH cargo: Str-Ii_IRES_VSVG-SBP-Halo

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    HaloTag
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIC-SM-HA

    Plasmid

    #111188

    Purpose

    High affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)

    Depositor

    Silvia Moreno

    Article

    Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

    Type

    Empty backbone

    Use

    Toxoplasma gondii expression

    Tags

    Spaghetti Monster HA Tag

    Expression

    Mutation

    Promoter

    Endogenous

    Available since

    Aug. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. Lenti-DAG1

    Plasmid

    #205149

    Purpose

    Overexpress DAG1

    Depositor

    Monkol Lek

    Insert

    Dystroglycan 1 (DAG1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Carries common missense variant c.41C>G (p.Ser…

    Promoter

    EF-1a

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWM_12x601_30bpLinker

    Plasmid

    #157789

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_30bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJ915

    Plasmid

    #69090

    Purpose

    Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

    Depositor

    Jennifer Doudna

    Article

    Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

    Insert

    cas9
    

    Use

    Tags

    MBP, hexa His, and two SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB6633

    Plasmid

    #106162

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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