We narrowed to 2,768 results for: SRS
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Plasmid#82697PurposeFor targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.DepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterhPGK, U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only
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SpCas9-NRCH-ABE8.17-m+V106W
Plasmid#198551PurposeExpresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-ABE8.17-m+V106W
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso A
Plasmid#137720PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoformDepositorInsertBRD4 long isoform (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECE-HA-AMPKbeta1 wt
Plasmid#31666DepositorInsertprotein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY30
Plasmid#84745PurposeExpresses huAsCpf1-P2A-puro and crRNA guideDepositorInsertshuAsCpf1
puromycin resistance gene
UseTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PPP1R12A wt
Plasmid#31669DepositorInsertProtein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
U6-pegRNA-H1-nick sgRNA-mCherry
Plasmid#176901PurposeExpresses prime editing guide RNA and guide RNA in mammalian cellsDepositorInsertsmCherry
gRNA scaffold
gRNA scaffold
UseAAVTagsExpressionMammalianMutationPromoterCAG, H1, and U6Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myc-RILPL1
Plasmid#201470PurposeFor overexpression in cultured cells to visualize RILPL1 protein interactions.DepositorInsertRILPL1 (RILPL1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC8 human IFN-gamma
Plasmid#17600DepositorInsertinterferon-gamma cDNA (IFNG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124434PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in DrosophilaDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso C
Plasmid#137721PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoformDepositorInsertBRD4 short isoform (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
-204 human IFN-gamma luc
Plasmid#17596DepositorInsertIFN gamma promoter (IFNG Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation-204 to +38 of promoterPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMP71-super-IL-2
Plasmid#174599PurposeExpresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia LabDepositorInsertextracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPing
Plasmid#47100PurposeExpresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.DepositorInsertsPing cDNA
mPing
hygromycin resistance gene
UsePlant expressionTagsGFPExpressionYeastMutationPromoterCaMV35s and StUbi3Available sinceSept. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pY31
Plasmid#84746PurposeExpresses huLbCpf1-P2A-puro and crRNA guideDepositorInsertshuLbCpf1
puromycin resistance gene
UseCRISPRTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-nosT
Plasmid#71272PurposeEntry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertnopaline synthase terminator
UseGatewayTagsExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CMV-Cas9-P2A-HygR
Plasmid#164133PurposeLentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cellsDepositorInsertSpCas9 (cas9 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HcRed
Plasmid#25892DepositorInserthcRed
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pY095
Plasmid#84744PurposeExpresses huLbCpf1-T2A-GFP and crRNA guideDepositorInsertshuLbCpf1
GFP
UseCRISPRTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn GAPDH
Plasmid#220918PurposeNeuronal specific expression of GAPDHDepositorInsertGAPDH (GAPDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only