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pACT2-μ3A

Plasmid

#198174

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-3 μ3A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

silent substitution in codon 225

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ3B

Plasmid

#198175

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-3 μ3B

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Expression

Insect

Promoter

hsp70

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBJ1-rat Beta2m

Plasmid

#121670

Purpose

Expresses rat β-2-microglobulin in mammalian cells

Depositor

Pamela Bjorkman

Article

Tesar et al Traffic. 2006 Sep;7(9):1127-42.

Insert

rat beta-2- microglobulin (B2m Rat)

Expression

Mammalian

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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NpT7-5-Erk2

Plasmid

#39230

Depositor

Melanie Cobb

Article

Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

Insert

Erk2 (Mapk1 Rat)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

Plasmid

#74306

Purpose

Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Inserts

TRPV4 (Trpv4 Rat)
ferritin (FTL and HTL1) (FTL, FTH1 Human)

Use

AAV and Cre/Lox

Tags

FLAG and p2A

Expression

Mammalian

Promoter

CMV (after cre recombination)

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-eBE-S3

Plasmid

#101093

Purpose

Expresses eBE-S3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S3

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

Plasmid

#26569

Depositor

Diane Lipscombe

Article

Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pENTR1A-HA-Jag1

Plasmid

#46052

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Entry vector

Tags

HA tag

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
NGL-1-myc/pEGFP-N1

Plasmid

#197314

Purpose

Plasmid expressing an antigen targeting Neuroligin-1

Depositor

Eunjoon Kim

Insert

Neuroligin-1 (Nlgn1 Rat)

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-oA ONE-GO

Plasmid

#204330

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-oA ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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N-WASP (WGP)

Plasmid

#11597

Depositor

Marc Kirschner

Article

Rohatgi et al J Cell Biol. 2000 Sep 18. 150(6):1299-31

Insert

N-WASP (Iqub Rat)

Expression

Mammalian

Mutation

contains amino acids 1-396

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-eGFP-SynaptoZip

Plasmid

#122525

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hSyn

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) rat C/EBP beta (LAP)

Plasmid

#12558

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta (Cebpb Rat)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179074

Purpose

Vector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TEF2p-Scarlet(rfp)-Link-2xPH

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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Rat Alpha2 delta-1 pMT2

Plasmid

#58726

Purpose

expression of rat alpha2 delta-1 in mammalian cells

Depositor

Annette Dolphin

Article

Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-GFP-LC3-mRuby3

Plasmid

#184898

Purpose

Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

LC3B

Tags

EGFP and mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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Fra-1

Plasmid

#187906

Purpose

Expresses Fra-1 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra1 (Fosl1 Rat)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Emerald-Lysosomes-20

Plasmid

#56476

Purpose

Localization: Lysosome Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

LAMP1

Tags

Emerald

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
p70 S6 kinase

Plasmid

#1892

Depositor

Joseph Avruch

Article

Weng et al Mol Cell Biol 1995 May;15(5):2333-40.

Insert

p70 alpha1 S6 kinase (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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