2,351 results

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pRB112

Plasmid

#180173

Purpose

pAAV construct GluN1WT (shRNA resistant)

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

WT

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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tdTurboRFP-Lysosomes-20

Plasmid

#58061

Purpose

Localization: Lysosome Membrane, Excitation: 553, Emission: 576

Depositor

Michael Davidson

Insert

LAMP1

Tags

tdTurboRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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EMSV-MRF4 (puro)

Plasmid

#14713

Depositor

Michael Rudnicki

Article

Sabourin et al Clin Genet. 2000 Jan . 57(1):16-25.

Insert

MRF4 (Myf6 Rat)

Expression

Mammalian

Available Since

April 2, 2007

Availability

Academic Institutions and Nonprofits only
pSuperGFP-cdc42-prenyl siRNA

Plasmid

#22715

Depositor

Alaa El-Husseini

Insert

cdc42 siRNA (Rac1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-SnFR-gamma8-minWPRE

Plasmid

#165498

Purpose

AAV for glutamate reporter fused to TARP gamma-8

Depositor

Andrew Plested

Article

Hao et al Elife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)

Use

AAV

Promoter

hSynapsin

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTrcHisA-rat-PTP1B

Plasmid

#35987

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

His and Xpress Epitope Tag

Expression

Bacterial

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) WT

Plasmid

#134695

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]

Plasmid

#26568

Depositor

Diane Lipscombe

Article

Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

contains exons a10, 18a, 31a, 37b, and 46. Del…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pEBG p130cas

Plasmid

#15001

Depositor

Raymond Birge

Article

Zvara et al Oncogene. 2001 Feb 22. 20(8):951-61.

Insert

p130cas (Bcar1 Rat)

Tags

GST

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only
LUC2 (L/Sec/deltaP)

Plasmid

#112145

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

Insert

Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)

Expression

Mammalian

Mutation

Cysteine 258 mutated to selenocysteine (U) and d…

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCR8-Magneto2.0

Plasmid

#74333

Purpose

For gateway cloning Magneto2.0 into destination vector

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0

Use

Gateway vector

Tags

Flag

Mutation

TRPV4: delta 760-871

Promoter

none

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP-shACTN4

Plasmid

#52679

Purpose

shRNA against α-actinin-4 with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Insert

ACTN4 (Actn4 Rat)

Use

RNAi

Tags

CMV-EGFP

Promoter

U6

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pOC2 Tubb3-GFP KI

Plasmid

#183432

Purpose

CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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cacnb1b (rat) in pMT2 vector

Plasmid

#107423

Purpose

Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

Insert

calcium channel beta1b auxillary subunit

Expression

Mammalian

Promoter

Ad MLP

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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Dronpa-Homer1-C-18

Plasmid

#57279

Purpose

Localization: Membrane Adaptor, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

Homer1 (Homer1 Rat)

Tags

Dronpa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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TSC2-3A (S1337A/S1341A/S1345A)

Plasmid

#29360

Depositor

Kunliang Guan

Article

Inoki et al Cell. 2003 Nov 26. 115(5):577-90.

Insert

TSC2 (Tsc2 Rat)

Expression

Mammalian

Mutation

S1337A/S1341A/S1345A (equivalent to S1381/S1385/S…

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
Cx43K258stop-msfGFP

Plasmid

#69023

Purpose

Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

V206K monomerized super folder GFP

Expression

Mammalian

Mutation

delete codons 258-381 and fuse in frame to monome…

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

Plasmid

#194015

Purpose

Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

Depositor

Larry Young

Article

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(Oxtr.1)
eGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV and u6

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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DHFR-GFP-th-GluA1

Plasmid

#173008

Purpose

For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-GFP-th-GluA1

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ASPM MTB

Plasmid

#27668

Depositor

Joseph Loturco

Article

Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

Insert

Abnormal spindle-like microcephaly associated (Aspm Rat)

Tags

GFP

Expression

Mammalian

Mutation

Contains amino‑terminal putative microtubule bind…

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only