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We narrowed to 9,280 results for: Pol

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Showing: 2701 - 2750 of 9280 results

  2. ATX3-77Q-R388G

    Plasmid
    #184251
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184251-plasmid-map-sequence-id-409179

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  6. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129048
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129048-plasmid-map-sequence-id-256223

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  7. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129041
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129041-plasmid-map-sequence-id-256196

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  8. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid
    #169181
    Purpose
    Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    No purification tag and nsp7-nsp8 fusion protein …
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169181-plasmid-map-sequence-id-334012

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  9. pAJ1

    Plasmid
    #138379
    Purpose
    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ
    Depositor
    Seth Darst
    Article
    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11
    Inserts
    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…
    Promoter
    T7
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138379-plasmid-map-sequence-id-275999

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  14. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

    Plasmid
    #129047
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA7 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA7 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129047-plasmid-map-sequence-id-256221

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  15. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

    Plasmid
    #129049
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA9 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129049-plasmid-map-sequence-id-256224

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  16. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid
    #129050
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA10 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129050-plasmid-map-sequence-id-256225

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  17. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid
    #129051
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA11 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129051-plasmid-map-sequence-id-256226

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  18. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

    Plasmid
    #129052
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA12 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA12 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129052-plasmid-map-sequence-id-256228

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  19. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid
    #129043
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA3 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA3 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129043-plasmid-map-sequence-id-256197

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  20. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129044
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA4 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129044-plasmid-map-sequence-id-256198

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  21. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

    Plasmid
    #129045
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA5 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129045-plasmid-map-sequence-id-256200

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  22. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid
    #129046
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA6 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129046-plasmid-map-sequence-id-256220

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  31. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid
    #52093
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52093-plasmid-map-sequence-id-80507

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  32. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid
    #52092
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52092-plasmid-map-sequence-id-80506

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  33. pGLU_82

    Plasmid
    #225783
    Purpose
    RS421 library variant 13550
    Depositor
    Nili Ostrov
    Insert
    13550_[abr]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225783-plasmid-map-sequence-id-447020

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  34. pGLU_100

    Plasmid
    #225813
    Purpose
    RS421 library variant 13550
    Depositor
    Nili Ostrov
    Insert
    13550_[Himar]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225813-plasmid-map-sequence-id-446992

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  35. pGLU_118

    Plasmid
    #225798
    Purpose
    RS421 library variant 13550
    Depositor
    Nili Ostrov
    Insert
    13550_[Tn5]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225798-plasmid-map-sequence-id-446919

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  36. pSLfa-PUb-MCS

    Plasmid
    #52908
    Purpose
    Aedes aegypti polyubiquitin promoter followed by MCS
    Depositor
    Zach Adelman
    Type
    Empty backbone
    Expression
    Insect
    Promoter
    Ae aegptyi polyubiquitin
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    52908-plasmid-map-sequence-id-82420

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  38. pOpen-T7gene1

    Plasmid
    #165505
    Purpose
    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T7 RNA Polymerase
    Use
    Synthetic Biology
    Available Since
    July 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165505-plasmid-map-sequence-id-338461

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  39. pOpen_taq

    Plasmid
    #153315
    Purpose
    Bacterial expression of Taq polymerase
    Depositor
    Drew Endy
    Insert
    Taq polymerase
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153315-plasmid-map-sequence-id-298774

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  40. pBS-3xpA

    Plasmid
    #176505
    Purpose
    3x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    3x SV40 polyA
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176505-plasmid-map-sequence-id-346556

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  41. pBS-2xpA

    Plasmid
    #176504
    Purpose
    2x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    2x SV40 polyA
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176504-plasmid-map-sequence-id-348023

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  42. pBS-4xpA

    Plasmid
    #176506
    Purpose
    4x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    4x SV40 polyA
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176506-plasmid-map-sequence-id-347136

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  43. L4437

    Plasmid
    #1652
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    RNAi
    Tags
    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, T7polN, T7…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1652-plasmid-map-sequence-id-262

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  44. pCAG-VSVL

    Plasmid
    #64085
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene
    Depositor
    Ian Wickersham
    Insert
    VSV polymerase
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64085-plasmid-map-sequence-id-429028

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  45. pJY4092

    Plasmid
    #113334
    Purpose
    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter
    Depositor
    Suzanne Sandmeyer
    Insert
    codon-optimized Hermes transposase
    Expression
    Yeast
    Available Since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113334-plasmid-map-sequence-id-218285

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  46. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

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  47. 371-A

    Plasmid
    #97026
    Purpose
    yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97026-plasmid-map-sequence-id-182505

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  48. pOpen-T5gene122

    Plasmid
    #165544
    Purpose
    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T5 DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165544-plasmid-map-sequence-id-339280

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  49. 371-U

    Plasmid
    #97036
    Purpose
    MYFQSNA-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    MYFQSNA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97036-plasmid-map-sequence-id-182502

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  50. pCAG-B19L

    Plasmid
    #59922
    Purpose
    Expression vector for Rabies virus SAD B19 RNA dependent RNA polymerase gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus RNA dependent RNA polymerase
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    59922-plasmid-map-sequence-id-94980

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Showing: 2701 - 2750 of 9280 results