We narrowed to 15,547 results for: pET
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Plasmid#190955PurposeTest the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP1
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP4_rpoA-U
Plasmid#190964PurposeTest the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP4
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP5_rpoA-U
Plasmid#190965PurposeTest the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP5
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM20-9S-DYW [rpoA]
Plasmid#176220PurposeExpression of TRX-9S-DYW [rpoA]DepositorInsertTRX-9S-DYW [rpoA]
UseSynthetic BiologyTagsTRX-HisPromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-GFP-9S [rpoA]
Plasmid#176221PurposeExpression of GFP-9S [rpoA]DepositorInsertGFP-9S [rpoA]
UseSynthetic BiologyTagsHis-GFPPromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM20-avi-9S-S2 [rpoA]
Plasmid#176223PurposeExpression of TRX-AviTag-9S-S2 [rpoA]DepositorInsertTRX-Avi-9S-S2 [rpoA]
UseSynthetic BiologyTagsTRX-His-AviTagPromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-CI2F
Plasmid#191254PurposeExpresses chymotrypsin inhibitor with an N-terminal MBP fusion proteinDepositorInsertChymotrypsin Inhibitor 2
TagsMaltose Binding ProteinExpressionBacterialAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-2xTS
Plasmid#162779PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-matNODALvar-HIS
Plasmid#115265PurposeFor bacterial recombinant protein production of the mature peptide of a human NODAL splice variantDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Sa-iPGM-bio
Plasmid#179523PurposeExpresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coliDepositorInsertS. aureus iPGM bio-6His
TagsHis tag and biotinylation sequenceExpressionBacterialPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Mo-iPGM-bio
Plasmid#179614PurposeExpresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coliDepositorInsertM. orale iPGM bio-6His
TagsHis tag and biotinylation sequenceExpressionBacterialPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Hp-iPGM-bio
Plasmid#179615PurposeExpresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coliDepositorInsertH. pylori iPGM bio-6His
TagsHis tag and biotinylation sequenceExpressionBacterialPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2-P229S-pET151
Plasmid#177871PurposeExpresses mouse Talin 1 F2-P229S domain in bacterial cellsDepositorInsertmouse Talin 1 F2 P229S mutant (Tln1 Mouse)
TagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2-P229L-pET151
Plasmid#177876PurposeExpresses mouse Talin 1 F2-P229L domain in bacterial cellsDepositorInsertmouse Talin 1 F2 P229L mutant (Tln1 Mouse)
TagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-pET151
Plasmid#177877PurposeExpresses mouse Talin 1 F2F3 domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 domain (Tln1 Mouse)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-Pep-H
Plasmid#185788PurposeEncodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-Pep4
TagsHis-tagExpressionBacterialPromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-TR-del1-40
Plasmid#180236PurposeExpresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364)DepositorInsertColicin E1
Tags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T and R domains (r…PromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-P110A
Plasmid#180237PurposeExpresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110DepositorInsertColicin E1
Tags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domain of ColE1 …PromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-bhaB5
Plasmid#167812PurposeExpresses His6- and MBP-tagged BhaB5 in E. coliDepositorInsertBhaB5
TagsHis6, MBPExpressionBacterialPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)_co_L-HicDH
Plasmid#185453PurposeCDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).DepositorInsertL-HicDH from Lactobacillus confusus DSM 20196
ExpressionBacterialPromoterT7Available SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-sfGFP
Plasmid#180756PurposepET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain
Plasmid#183387PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertredesigned Caspase-4 (AA94-377) (CASP4 Human)
TagsHis-TEVExpressionBacterialMutationANR261ENP, D315H, M318K, T352KPromoterT7Available SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2333_Tier3(Lenti_inverse)-YPet_Puro
Plasmid#169664PurposeTier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)DepositorInsertPRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPhCMV / RPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2361_Tier3(SB)-rtTA-YPet_PuroR
Plasmid#169657PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2363_Tier3(PB)-rtTA-YPet_PuroR
Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-SU(Z)12(624-900)-H6
Plasmid#183772PurposeExpress of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.DepositorInsertSU(Z)12 residues 624-900 (Su(z)12 Fly)
TagsHis tag at the C-terminusExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-Dmp68_B
Plasmid#145966PurposeBacterial Expression of Dmp68DepositorInsertDmp68 (Rm62 Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)
Plasmid#178034PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deletedPromoterT7LacIAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-CPL2/pET28a
Plasmid#172095PurposecDNA of CER11/CPL2 cloned into pET28a for protein expressionDepositorAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28_6xHis-PARP1delZn1-L713F
Plasmid#173937PurposeBacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn1-L713F (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 1-96; L713F, V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3-L713F
Plasmid#173934PurposeBacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn3-L713F (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 214-373; L713F, V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G871L
Plasmid#174798PurposeBacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A870L
Plasmid#174797PurposeBacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-K893I
Plasmid#174799PurposeBacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-K77R
Plasmid#174813PurposeBacterial expression of inactive ALC1 (K77R disrupts ATP binding)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-E175Q
Plasmid#174814PurposeBacterial expression of inactive ALC1 (E175Q disrupts ATP binding)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-43
Plasmid#172759PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-43
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-35
Plasmid#172755PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-35
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-19
Plasmid#172748PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-19
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-3
Plasmid#172740PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-3
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-12
Plasmid#172744PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-12
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-14
Plasmid#172745PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-14
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-11a HIV-1 P6
Plasmid#154201PurposeBacterial expression of Gag P6 from HIV-1 HXB2 strainDepositorInsertGag P6 protein from HIV-1 clone HXB2
TagsGB1-6xHis TagExpressionBacterialPromoterT7Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-dre4
Plasmid#170440PurposeExpress drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.DepositorInsertdre4 (dre4 Fly)
Tags6xHis and V5ExpressionBacterialMutationDeleted amino acids 1-20PromoterT7Available SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1-4H pET151
Plasmid#159386PurposeExpresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 (TLNRD1 Human)
Tagshis-tag, TEV cleavage siteExpressionBacterialMutationTLNRD1 4-helix domain (residues 143-273)PromoterT7Available SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30 W96A-GLTP
Plasmid#170734PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a Ec-iPGM-bio
Plasmid#162575PurposeExpresses E. coli iPGM containing a C-terminal biotinylation site and His tagDepositorInsertE. coli iPGM bio-6His
Tags6His tag, T7 tag, biotinylation sequence, and thr…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-Sec22b
Plasmid#168029PurposeExpresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cellsDepositorInserteCFP-Sec22b(A131-H190)-mVenus
TagsHisx6 and mycExpressionBacterialAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-VAMP8
Plasmid#168028PurposeExpresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cellsDepositorInserteCFP-VAMP8(G7-R67)-mVenus
TagsHisx6 and mycExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only