We narrowed to 25,852 results for: LARS

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pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113343

Purpose

Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

p300core-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-nucl

Plasmid

#113915

Purpose

Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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Opto-ACTU−

Plasmid

#198869

Purpose

Optogenetic recruitment construct to increase inner membrane surface charge

Depositor

Peter Devreotes

Article

Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

Insert

Rad17 polyanionic tail (-14)

Use

Synthetic Biology

Tags

CRY2PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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ML1Nmut_in_pEGFP-C1

Plasmid

#67796

Purpose

Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_SARS-CoV-2_Sdel18

Plasmid

#207307

Purpose

Mammalian expression of SARS2 coronavirus S protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SARS-CoV2_S (S SARS2 coronavirus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-memRFP

Plasmid

#224564

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

mRFP1

Use

Unspecified

Tags

Membrane localization signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

Plasmid

#191338

Purpose

Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP

Plasmid

#34631

Depositor

Wolfhard Almers

Article

Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

Insert

rat Syntaxin1a (Stx1a Rat)

Tags

meGFP

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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Sar1b-mOrange2

Plasmid

#166899

Purpose

mammalian expression of Sar1b tagged with mOrange2

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mOrange2

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd3

Plasmid

#42255

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 3 (Fzd3 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
His-GFP-Rabphilin

Plasmid

#170865

Purpose

Mammalian expression of His-GFP-Rabphilin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rabphilin-3a (RPH3A Bovine)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM253 - sgRNA mosTI hygroR

Plasmid

#159827

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI hygroR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mKO2-pBAD

Plasmid

#54555

Purpose

Localization: Protein Expression Vector , Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

6xHis and mKO2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3-GFPemd

Plasmid

#110210

Purpose

Labels cells in the late osteoblast lineage.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPemerald

Tags

None

Expression

Mammalian

Promoter

2.3 kb rat type 1 collagen + first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rab3a

Plasmid

#170863

Purpose

Mammalian expression of His-GFP-Rab3a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-3a (Rab3a Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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Shp2 biosensor

Plasmid

#134346

Purpose

FRET biosensor. To visualize Shp2 activity by FRET

Depositor

Yingxiao Wang

Article

Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

Insert

Shp2 Biosensor

Expression

Mammalian

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pYSD085

Plasmid

#212922

Purpose

Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

649 Stalk

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMM135

Plasmid

#127216

Purpose

BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

Plasmid

#134857

Purpose

Expresses EGFP-tagged synaptic vesicle marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 3C

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)

Plasmid

#191144

Purpose

Expression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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