We narrowed to 15,984 results for: grna

1. pT7-AsCas12a_crRNA-site4 (MSP3511)

   Plasmid

   #160139

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSicoR human DGCR8-2

   Plasmid

   #14770

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   DGCR8 shRNA (DGCR8 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pSicoR human Drosha2

   Plasmid

   #14767

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   Drosha shRNA (DROSHA Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pSuper mouse Drosha1

   Plasmid

   #14780

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   Drosha shRNA (Drosha Mouse)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pSuper mouse DGCR8-1

   Plasmid

   #14772

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   DGCR8 shRNA (Dgcr8 Mouse)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pX459-sg-mGreenLantern/EGFP

   Plasmid

   #179913

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern/EGFP sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAC155-pCR8-sgExpression

   Plasmid

   #49045

   Purpose

   U6-driven sgRNA expressing cassette on a gateway donor vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-hU6-mU6-BlasGO

   Plasmid

   #136906

   Purpose

   Lentivirus for base editing activatable Blasticidin resistance gene expression in mammalian cells. All in one vector with sgBlasGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   BlasCyGO
   

   Use

   Lentiviral

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

   Plasmid

   #172225

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSAG1::CAS9-U6::sg290860-6

    Plasmid

    #59855

    Purpose

    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

    Depositor

    David Sibley

    Article

    Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

    Insert

    CRISPR sg290860-6
    

    Use

    CRISPR; ; toxoplasma gondii

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSuper mouse Dicer3

    Plasmid

    #14778

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Dicer shRNA (Dicer1 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

12. Geph CR shRNA

    Plasmid

    #121068

    Purpose

    shRNA targeting the coding region of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDA_186

    Plasmid

    #133458

    Purpose

    U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L40C-CRISPR.EFS.dTomato

    Plasmid

    #89392

    Purpose

    Lentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter driven

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSR-puro-Mff1 shRNA

    Plasmid

    #37247

    Depositor

    Christopher Counter, David Kashatus

    Article

    Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

    Insert

    Mff1 (MFF Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pSicoR-mCh-Oct4i

    Plasmid

    #21906

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Insert

    Oct4i
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMBA323

    Plasmid

    #214751

    Purpose

    To create delta(glmS,nadE) working strains and maintaining their viability

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSicoR-RNF40i1

    Plasmid

    #59594

    Purpose

    Expression of shRNA against human RNF40

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    RNF40 (RNF40 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

19. LentiCRISPRv2puro-Tyr

    Plasmid

    #170293

    Purpose

    A knock-out vector for the mouse tyrosinase.

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pV1435

    Plasmid

    #111439

    Purpose

    Solo vector pV1382 + sgCgADE2

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgCgADE2
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge