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pECFP-N2-COL1A1

Plasmid

#66603

Purpose

Expresses procollagen 1 alpha 1 as a CFP fusion

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

Insert

COL1A1 (COL1A1 Human)

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pWZLneo Flag mPTIP

Plasmid

#15558

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PTIP (Paxip1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pRET.IS.IRES-EGFP N1

Plasmid

#1833

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
IronFist

Plasmid

#243006

Purpose

Genetically encoded fluorescent iron reporter

Depositor

Roland Malli

Article

Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

Inserts

Hemerythrin-like (FBXL5 Human)
T2A
mCherry

Use

Gateway Cloning

Tags

mNeonGreen

Expression

Mammalian

Promoter

human phosphoglycerate kinase (hPGK)

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63-SBP-mCherry

Plasmid

#222326

Purpose

Synchronize the trafficking of CD63 from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1_puro_shNRF2-2

Plasmid

#230087

Purpose

shRNA silencing human NRF2 gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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JN202: pMVP/SB/Blast-DEST

Plasmid

#121864

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGL2-Skp2 promoter-luciferase

Plasmid

#81119

Purpose

Reporter activity of SKP2 gene promoter

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (Skp2 Mouse)

Use

Luciferase

Tags

luciferase

Mutation

contains the region from 1_kb upstream of transcr…

Promoter

SKP2

Available Since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only
pLPCX-Cx43-FS-IRES-GFP

Plasmid

#65438

Purpose

Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

frameshift (FS) mutation 35delA

Promoter

CMV

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Smad5 CDS

Plasmid

#61800

Purpose

pcDNA3.1(+) with full length mouse Smad5 CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Smad5 CDS (Smad5 Mouse)

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pESC-URA-hPOLISc

Plasmid

#60819

Purpose

to express human Pol iota recoded to yeast codons in yeast

Depositor

Youri Pavlov

Article

Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

Insert

POLI (POLI Human)

Tags

c-myc

Expression

Yeast

Mutation

optimized to yeast codon usage

Promoter

GAL1

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHM6g.AQ_1489

Plasmid

#11539

Depositor

Sung-Hou Kim

Article

Liu et al Proteins. 2003 Nov 1. 53(2):326-8.

Insert

tRNA methyltransferase

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pCMV3TAG8li-hSTT3A-3FLAG

Plasmid

#159140

Purpose

Hu STT3A -flag mammalian expression vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

STT3 oligosaccharyltransferase complex catalytic subunit A (STT3A Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L

Plasmid

#89610

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L (DOT1L Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-hARΔ142–337

Plasmid

#89106

Purpose

Mammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1

Depositor

Elizabeth Wilson

Article

Titus et al PLoS One. 2012;7(1):e30192. doi: 10.1371

Insert

hAR delta 142-337 (AR Human)

Expression

Mammalian

Mutation

hAR delta 142-337

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag Rab1a N124I

Plasmid

#46778

Purpose

expression of N124I Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (Rab1a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N124I cannot stably bind guanine nucleotide

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pLEXSY-hyg2.1_LtIFT43_Flag

Plasmid

#194433

Purpose

Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae

Depositor

Alan Brown

Article

Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.

Insert

Intraflagellar transport complex subunit 43

Use

Leishmania tarentolae expression

Tags

3X Flag and Linker

Promoter

L.donovani promoter elements

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2

Plasmid

#202761

Purpose

Encodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harbor

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

NEUROG2-p2A-ATOH7-p2A-ISL1-p2A-POU4F2 (NEUROG2, ATOH7, ISL1, POU4F2 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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cyt-GafD-mTurboID-V5

Plasmid

#184640

Purpose

Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

Depositor

Charlie Fehl

Article

Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

Insert

cyt-GlycoID-V5

Tags

Nuclear export sequence and V5 tag

Expression

Mammalian

Mutation

GafD -> expressed aa22-178 ; BirA* -> expre…

Promoter

CMV

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

Plasmid

#176627

Purpose

TF B self-activation cassette in MultiFate-2.2 and MultiFate-3

Depositor

Michael Elowitz

Article

Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

Insert

TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

Use

Synthetic Biology

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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