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161,813 results

Showing: 11021 - 11040 of 161813 results

  1. gWiz_BM40-vMAT2-VL-hIgG1-CL

    Plasmid
    #218443
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vMAT2 Fab domain
    Depositor
    Axel Brunger
    Insert
    vMAT2-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218443-plasmid-map-sequence-id-431083

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  2. MRGPRX1-DuET

    Plasmid
    #213344
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MRGPRX1 (MRGPRX1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213344-plasmid-map-sequence-id-430872

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  9. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  13. Tet-pLKO-FOXM1-shRNA-#1

    Plasmid
    #89498
    Purpose
    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    June 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    89498-plasmid-map-sequence-id-181255

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  17. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI-D10R-E48R
    Use
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60367-plasmid-map-sequence-id-456413

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  18. SIVh

    Plasmid
    #81091
    Purpose
    Single Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81091-plasmid-map-sequence-id-164494

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Showing: 11021 - 11040 of 161813 results