159,461 results

1. pDONR221_SLC2A2

   Plasmid

   #132126

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC2A2 (SLC2A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCiES

   Plasmid

   #60010

   Purpose

   Foamy virus helper plasmid expressing Env, driven by a CMV promoter. Ampicillin resistant.

   Depositor

   David Russell

   Article

   Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

   Insert

   Env (foamy virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Neo-M2rtTA

   Plasmid

   #60843

   Purpose

   AAVS1 donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

   Inserts

   M2rtTA
   Neo
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. MS2-Snapf

   Plasmid

   #65860

   Purpose

   Expresses RNA-binding protein MS2 with fast SNAP fusion tag

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

   Inserts

   MS2
   Fast SNAP
   

   Use

   Tags

   HA and NLS

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

5. mKeima-Red-C1

   Plasmid

   #54546

   Purpose

   Localization: C1 Cloning Vector, Excitation: 440, Emission: 620

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mKeima-Red

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUC01

   Plasmid

   #177204

   Purpose

   chromogenic reporter for gene knockout in Streptomyces

   Depositor

   Wenjun Guan

   Article

   Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

   Insert

   SaindC
   

   Use

   Synthetic Biology

   Tags

   NONE

   Expression

   Bacterial

   Mutation

   NONE

   Promoter

   TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBR1k-GFPuv

   Plasmid

   #106382

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. tdEos-Integrin-Beta1-N-18

   Plasmid

   #57627

   Purpose

   Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Insert

   Integrin-Beta1 (ITGB1 Human)
   

   Use

   Tags

   tdEos

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pWT019a

   Plasmid

   #107893

   Purpose

   W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PTetO-sd2U-Cas9, PLacO-sgRNA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV.5’-LMNA-eGFP-KI

    Plasmid

    #170545

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for LMNA knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExp-MBP

    Plasmid

    #112568

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-MBP and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-vioABECD

    Plasmid

    #66535

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Consensus T7

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Dronpa-H3.3-N-14

    Plasmid

    #57278

    Purpose

    Localization: Nucleus/Histones, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Insert

    H3.3 (H3f3b Mouse)
    

    Use

    Tags

    Dronpa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. PS-CFP2-CENPA-N-22

    Plasmid

    #57218

    Purpose

    Localization: Nucleus, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    CENPA (CENPA Human)
    

    Use

    Tags

    PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pUC19-Mu-switch-R-loop

    Plasmid

    #134899

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mouse IgH switch repeat region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mGeos2-VEL-PMP-N-10

    Plasmid

    #57557

    Purpose

    Localization: Peroxisomes, Excitation: 503 / 569, Emission: 514 / 581

    Depositor

    Michael Davidson

    Insert

    PMP (PXMP2 Human)
    

    Use

    Tags

    mGeos2-VEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. reporter-gT1

    Plasmid

    #47320

    Purpose

    TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    minCMV-dTomato_pA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSKI074

    Plasmid

    #11571

    Depositor

    Detlef Weigel

    Article

    Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

    Insert

    CaMV 35S enhancers
    

    Use

    Activation tagging

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. beta-arrestin2 mYFP

    Plasmid

    #36917

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCiGSΔΨ

    Plasmid

    #108312

    Purpose

    Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.

    Insert

    gag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pCiGSΔΨ is a newer version of the pCiGS Gag expre…

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge