177,604 results
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Plasmid#79700PurposeThis plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pHR_AXP_hrGFP
Plasmid#84613PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locusDepositorPublicationInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hsgk1
Plasmid#83432Purposefull length human sgk1 with Flag tagDepositorAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pDNR-SpCas9-Gem
Plasmid#80424PurposeUsed for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joiningDepositorInsertSpCas9-Gem
UseCRISPRTagsGemininMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTag-BFP-C-h-Rab11a
Plasmid#79805PurposeExpress TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab11a (RAB11A Human)
TagsTagBFPExpressionMammalianPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA
Plasmid#74513PurposeAAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.DepositorInsertEGFP-p2A-EGFP-f
UseAAVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-V2-ORF
Plasmid#73636PurposeGateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).DepositorTypeEmpty backboneTagsVenus fragment 2 (V2)ExpressionMammalianPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNICKclos2.0
Plasmid#73228PurposeGenome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9 nickase
sgRNA to xylR
UseE.coli - clostridium shuttle vectorMutationD10APromoterPj23119 and PthlAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3 91-710
Plasmid#75140PurposeExpresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
TagsFlagExpressionMammalianMutationfragment encoding amino acids 91-710PromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZW1-FCS-circRNA
Plasmid#73449PurposeExpression of circular RNADepositorAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIP-VEGFA121
Plasmid#73017PurposeRetroviral expression of human VEGFA121DepositorAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DH-BB(5'SIN;TE12ORF)
Plasmid#72309PurposeDefective Helper to produce neurotropic, propagation-incompetent Sindbis virusDepositorInsertDH-BB(5'SIN;TE12ORF)
UseSindbis virus helper constructAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-EF1-DIO-tdTomato
Plasmid#72254PurposeExpress tdTomato under EF-1 promoter when Cre is expressed by another vectorDepositorInserttdTomato
UseCre/Lox and LentiviralExpressionMammalianPromoterelongation factor-1Available sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAM-kan-R4R3
Plasmid#71275PurposeBinary destination vector for plant transformation. Kanamycin resistance.DepositorTypeEmpty backboneUseGateway CloningAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEGFP-S727A-STAT3
Plasmid#71446PurposeRetroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.DepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGZ108 (pFA6a-3FLAG-MNase-kanMX6)
Plasmid#70231PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY011 (pcDNA3.1-hLb2Cpf1)
Plasmid#69983PurposeExpresses humanized Lb2Cpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthLb2Cpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GRM2-Tango
Plasmid#66388PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGRM2 (GRM2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by