165,744 results

1. YFP-NOP56-C3

   Plasmid

   #134536

   Purpose

   Expresses YFP-NOP56 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   NOP56 (NOP56 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. H3K9me3Probe_FullLength

   Plasmid

   #186463

   Purpose

   Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

   Depositor

   Chongli Yuan

   Article

   Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

   Insert

   dimeric CDY1 (CDY1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAJE-Ma pylRS nitroY/haloY-F5

   Plasmid

   #225684

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

   Depositor

   Ryan Mehl

   Inserts

   M. alvus PylRS 3-NY/HaloY F5
   M. alvus Pyl-tRNA(6)
   

   Use

   Methanomethylophilus alvus

   Mutation

   CTG125CTT, N166A, V168S, A223C, W239R

   Promoter

   GlnS and Lpp

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LifeAct-GFP-cytoB5RR

   Plasmid

   #182577

   Purpose

   Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   LifeAct-GFP-cytoB5RR
   

   Tags

   LifeAct and cytoB5RR

   Expression

   Mammalian

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUAST-attB-yki:V5-S111A, S168A, S250A

   Plasmid

   #85620

   Purpose

   Expresses V5 tagged triple mutant yki in Drosophila.

   Depositor

   Kenneth Irvine

   Article

   Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

   Insert

   yki-V5-S111A,S168,S250A
   

   Tags

   V5

   Expression

   Insect

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EFS-MCP-HALOnls

   Plasmid

   #121937

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   MCP-HALOnls
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

   Plasmid

   #136904

   Purpose

   Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   mScarletGo-p2a-Blas
   

   Use

   Lentiviral

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGFP-Cytochrome C

   Plasmid

   #41181

   Depositor

   Douglas Green

   Article

   Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

   Insert

   Cytochrome C (CYCS Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-pro-Il1b-C-Flag

   Plasmid

   #75131

   Purpose

   Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   interleukin 1 beta (Il1b Mouse)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHM130

    Plasmid

    #225529

    Purpose

    Encodes destablized GFP under IBRE4 promoter, and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    GFP
    BFP
    

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Tight-Puro_MYRF

    Plasmid

    #64843

    Purpose

    A lentiviral vector for the expression of mouse MYRF under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    MYRF (Myrf Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. px330_Rosa_sgRNA

    Plasmid

    #97007

    Purpose

    Expresses Cas9 and Rosa26 locus specific sgRNA

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Insert

    Rosa26 sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICE-EGFP-FLAG-PSMD2

    Plasmid

    #161917

    Purpose

    Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCXIP-INHBA

    Plasmid

    #73015

    Purpose

    Retroviral expression of human activin A

    Depositor

    Michael Grusch

    Insert

    INHBA (INHBA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF3476

    Plasmid

    #144952

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SNAI1 (SNAI1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-PLXNB2

    Plasmid

    #86237

    Purpose

    Lentivirus for expression of human Plexin-B2

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    full length PLXNB2, CRISPR resistant

    Promoter

    human PGK promoter

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD33-Gm

    Plasmid

    #65098

    Purpose

    bacterial expression vector with Gentamycin selection, based on pBAD33

    Depositor

    Juan Tomas

    Article

    Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDB179-TurboPETase

    Plasmid

    #218693

    Purpose

    Encodes codon optimized TurboPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TurboPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSW181 - GR-LhG4

    Plasmid

    #115983

    Purpose

    entry vector for GreenGate cloning method, CDS

    Depositor

    Jan Lohmann

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    GR-LhG4
    

    Use

    Unspecified

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

    Plasmid

    #191537

    Purpose

    Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-ddMisCas13b-SNAPf-NLS-3xFlag
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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