We narrowed to 13,970 results for: GAN

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iGFP-beta-actin

Plasmid

#231553

Purpose

Mammalian expression of human beta actin fused intramolecularly to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

ACTB (ACTB Human)

Tags

msfGFP (intramolecular)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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P521_VSX2_p2A-h2b_mRuby3

Plasmid

#239104

Purpose

Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

Depositor

Karl Wahlin

Article

Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

Insert

VSX2 (VSX2 Human)

Use

CRISPR

Tags

-p2A-h2b-mRuby3

Expression

Mammalian

Promoter

Endogenous VSX2 promoter

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut2

Plasmid

#180425

Purpose

mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMIH-mRuby2-BAK

Plasmid

#111627

Purpose

Fluorescent fusion protein used to visualise mouse BAK, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

BAK (Bak1 Mouse)

Use

Retroviral

Tags

mRuby2

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TRIP12-V2_A755-A841

Plasmid

#194759

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Article

Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

Insert

TRIP12 (TRIP12 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-CMV-PE2-IRES-ZeoR

Plasmid

#207352

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

Prime editor 2 (PE2) enzyme

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSLED.NPL

Plasmid

#193014

Purpose

Neuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)

Depositor

Jonathan Ling

Article

Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

Insert

Bichromatic reporter (EGFP and dsRed-Express2)

Use

AAV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-puro

Plasmid

#167817

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-TDP-43 M337V

Plasmid

#162609

Purpose

Allows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

M337V

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#1

Plasmid

#174152

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgPnpt1-1#

Plasmid

#234769

Purpose

knockout Pnpt1

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Pnpt1 (Pnpt1 )

Use

Lentiviral

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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R713-M85-303: CMV51p> Halotag-Hs.KRAS4a

Plasmid

#159680

Purpose

Mammalian protein expression of KRAS4a-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.KRAS4a (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-2

Plasmid

#118020

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-2 (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEx-iSeroSnFR

Plasmid

#128486

Purpose

Fluorescent reporter for serotonin (conditional + membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Has Service

AAV9

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, PDGFR, and cpsfGFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Ad:2CMV_A2R2-N25Ctr

Plasmid

#122243

Purpose

Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

Depositor

Corey Neu

Article

Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

Inserts

C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
Actinin alpha 2 (Actn2 Mouse)

Use

Adenoviral

Tags

Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

Expression

Mammalian

Mutation

deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

Promoter

CMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-SpyTag-Flag-Lbr-V5-mCherry

Plasmid

#235094

Purpose

Lbr mCherry fusion protein without MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-SpyTag-Flag-Lbr-V5-mCherry (LBR Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-4

Plasmid

#118022

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-4 (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-AMBRA1-3xFLAG

Plasmid

#174159

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1A

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-1

Plasmid

#118019

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-1 (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

Plasmid

#207355

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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