We narrowed to 24,467 results for: ina
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Plasmid#157765PurposeExpression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoterDepositorInsertAURKA (AURKA Human)
TagsGFPExpressionMammalianMutationpromoter CMV replaced by AurkA promoterPromoterAURKA minimalAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113702PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV, CRISPR, and Mouse TargetingTagsKASHAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shGLS
Plasmid#110319PurposeshGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2DepositorInsertGLS Glutaminase (GLS Human)
UseLentiviral and RNAiExpressionMammalianPromoterRNA polymerase III promoter for human U6 snRNA fo…Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL3-deltaTM
Plasmid#109025Purposestable lentiviral expression of cDNADepositorInsertATL3-deltaTM (ATL3 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 T230A 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86023PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationLacks mucin-like domain: Changed threonine 230 to…PromoterCMV IEAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86024PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationLacks mucin-like domain: Changed aspartic acid 63…PromoterCMV IEAvailable SinceApril 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-MutS2-C160S
Plasmid#85668PurposeExpression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
TagsGFPExpressionMammalianMutationC160S and I292Q and V295QPromoterCMVAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Akt3 WT-2X Flag
Plasmid#87607Purposeexpresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 proteinDepositorInsertAkt3 isoform 2 (AKT3 Human)
UseZebrafish (danio rerio) expressionTags2 tandem flag tags and His-Halo-TevExpressionMammalianMutationAkt3 isoform 2PromoterSP6Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
GIV-FLAG S245A (SA)
Plasmid#86095PurposeExpression GIV in mammalian cellDepositorAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
GIV-FLAG S245D (SD)
Plasmid#86096PurposeExpression GIV in mammalian cellDepositorAvailable SinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-FRB-W2027F
Plasmid#73341PurposePoint mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTORDepositorInsertFKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
TagsGST-taggedExpressionBacterialMutationFRB domain only aa2015-2114; W2027FAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
NG1237 pCI-TPI-PTC160-4H
Plasmid#65804PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
Tags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 160PromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIS1 mIL6R
Plasmid#60797PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and mutated miR-155 sitesDepositorInsertIL6R 3'UTR and mutated miR-155 binding site (IL6R Human)
UseLuciferaseExpressionMammalianMutationmutated miR-155 binding sitePromoterthymidine kinase (HSV-TK promoter)Available SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain
Plasmid#58725PurposeExpresses GFP-Tagged CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
TagsGFP and MycExpressionYeastPromoterPHO5Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMiR-E6AP-3'UTR-miR-375-mut
Plasmid#53695PurposeLuciferase reporter assay for E6AP 3'UTR with point mutation on miR-375 binding siteDepositorInsertUBE3A 3'UTR (UBE3A Human)
UseLuciferaseMutationMutation on miR-375 binding site on E6AP 3'U…Available SinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP10-bio
Plasmid#47719PurposeExpresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP10
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
ASP-bio
Plasmid#47745PurposeExpresses enzymatically monobiotinylated full-length ASP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised ASP
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RH1-bio
Plasmid#47748PurposeExpresses enzymatically monobiotinylated partial RH1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RH1 (partial)
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RAP1-bio
Plasmid#47794PurposeExpresses enzymatically monobiotinylated full-length RAP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RAP1
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only