159,481 results

1. HA-14-3-3 epsilon

   Plasmid

   #116886

   Purpose

   Expresses HA-tagged human 14-3-3 epsilon in mammalian cells

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Article

   Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

   Insert

   14-3-3 epsilon (YWHAE Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Gal4BD-CRY2(535)

   Plasmid

   #75369

   Purpose

   Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Gal4BD

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS160

   Plasmid

   #194483

   Purpose

   synDHBR Cassette

   Depositor

   Ahmad Khalil

   Article

   Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

   Insert

   synDHBR Cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBN42

   Plasmid

   #86713

   Purpose

   C. elegans injection marker

   Depositor

   Peter Askjaer

   Article

   Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

   Insert

   Pmyo-3::GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Pak3(60-113)/S74A/F84A-mCherry-C1

   Plasmid

   #29676

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Nature. 2011 Mar 20. ():.

   Insert

   Pak3 (PAK3 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   aa60-113 only; S74A F84A

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. FGFR1-optoDroplet

   Plasmid

   #111509

   Purpose

   For optogenetic clustering of FGFR1

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

   Insert

   FGFR1 (FGFR1 Human)
   

   Use

   Lentiviral

   Tags

   Cry2, FUS, Fusion Red, and Myristoylation sequenc…

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_NRAS_p.Q61R

   Plasmid

   #81652

   Purpose

   Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Q61R

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP

   Plasmid

   #165830

   Purpose

   EGFP green fluorescent protein CDS

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB3cK_pGAP_23*_pTEF_Cas9

   Plasmid

   #104909

   Purpose

   hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLRT

    Plasmid

    #78234

    Purpose

    Expresses Murine Leukemia Reverse Transcriptase (MLRT)

    Depositor

    Christopher Voigt

    Article

    Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

    Insert

    Murine leukemia reverse transcriptase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    Aug. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_SOX10_P2A_Hygro_Barcode

    Plasmid

    #120479

    Purpose

    Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162378

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTEF:ATP

    Plasmid

    #92179

    Purpose

    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    

    Use

    Integration and expression into yeast.

    Tags

    Yeast codon optimized mseCFP (donor) and cp173-mV…

    Expression

    Yeast

    Mutation

    Promoter

    TEF1p

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPX4

    Plasmid

    #38797

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GPX4 (GPX4 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    SeCys73 -> Gly

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-eGFP-C1-VPS35

    Plasmid

    #163622

    Purpose

    human VPS35 cDNA C-terminally tagged with eGFP

    Depositor

    Josef Kittler

    Article

    Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

    Insert

    human VPS35 (VPS35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-mRuby2-Gephyrin.FingR-IL2RGTC

    Plasmid

    #125696

    Purpose

    Global labeling of inhibitory synapses (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    mRuby2-Gephyrin.FingR-IL2RGTC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS1995

    Plasmid

    #49125

    Purpose

    AAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple94-iCre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GPR88

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pVITRO1-dV-IgG1/κ

    Plasmid

    #52213

    Purpose

    Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin kappa constant region
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1-E988K

    Plasmid

    #173945

    Purpose

    Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-E988K (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A, E988K

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA flag PPAR gamma

    Plasmid

    #8895

    Purpose

    Mammalian expression of flag-tagged PPAR gamma

    Depositor

    Bruce Spiegelman

    Article

    Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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