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psiCHECK-2-Map3k1-3'UTR

Plasmid

#61776

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR

Use

Luciferase

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
SepOTSθ

Plasmid

#68289

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 2xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pBGO

Plasmid

#62671

Purpose

Donor vector for mFTH1 knock-in via landing pad

Depositor

Masato Ohtsuka

Publication

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FTH1

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Fhod1-3'UTR

Plasmid

#61773

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR

Use

Luciferase

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR

Plasmid

#61783

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR

Use

Luciferase

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pUC-dirTopo-AU1-aGFP

Plasmid

#61973

Purpose

A directional cloning vector of PCR-amplified cDNAs for mammalian expression

Depositor

Hiroshi Udo

Inserts

lacZ alpha
EGFP

Expression

Mammalian

Mutation

It contains silent mutations

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LI D-E C-NLS c2

Plasmid

#54428

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

NLS of SV40 large T antigen

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D destab GFP c1

Plasmid

#54454

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

destablized GFP

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP Δcen mCherry

Plasmid

#45227

Depositor

Michael Lampson, Susanne Lens

Publication

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

vsv Cenp-B INCENP Δcen mCherry

Mutation

CEN box deleted from INCENP (aa1-46)

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-eLACCO3H

Plasmid

#256052

Purpose

Biosensor for extracellular L-lactate

Depositor

Yusuke Nasu

Publication

Liu et al bioRxiv October 5, 2026

Insert

eLACCO3H

Expression

Mammalian

Available since

Oct. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA-eLACCO3M

Plasmid

#256054

Purpose

Biosensor for extracellular L-lactate

Depositor

Yusuke Nasu

Publication

Liu et al bioRxiv October 5, 2026

Insert

eLACCO3M

Expression

Mammalian

Available since

Oct. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pOS1T-MbPylRS4ThzA

Plasmid

#253668

Purpose

Production of engineered aaRS/tRNA pair for incorporation of 4ThzA

Depositor

Alexandria Deliz Liang

Publication

Natter Perdiguero et al J Am Chem Soc. 2026 Apr 8;148(13):13619-

Inserts

MbPylRS-4ThzA
MmPylT_CUA

Expression

Bacterial

Available since

June 25, 2026

Availability

Academic Institutions and Nonprofits only

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pBK lpp MaPylT glnS AST

Plasmid

#255035

Purpose

Plasmid encoding mutant M alvus pyrrolysyl synthetase-AST and tRNA for o-nitrophenylalanine

Depositor

Abhishek Chatterjee

Publication

Soni et al ACS Cent Sci. 2024 May 20;10(6):1211-122

Inserts

M. alvus pyrrolysyl synthetase-AST
tRNAMaPyl

Expression

Bacterial

Mutation

L125A, N166S, V168T

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mScarlet-I-QtSig

Plasmid

#236775

Purpose

Expresses the red fluorescent protein mScarlet-I fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Publication

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

HA_DD-N_mScarlet-I_QtSig

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGY214

Plasmid

#239805

Purpose

Express EGFP and mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Publication

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBla_GFP

Plasmid

#223806

Purpose

inducible expression of low-copy Bla and and constitutive expression of GFP

Depositor

Lingchong You

Publication

Ma et al Nat Commun. 2024 Sep 27;15(1):8337. doi:

Inserts

superfolder GFP
Bla

Use

Unspecified

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pProUSER13F4C

Plasmid

#178023

Purpose

E. coli - B. subtilis nicking vector pProUSER13F4C. Ap-BBR1; ΔsigF-insertion-site; Km; manR-PmanA

Depositor

Sheila Jensen

Publication

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Pman

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pJM220-PhoA-mNeongreen-J23101-mScarletI

Plasmid

#240201

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A strong Anderson promoter (J23101) drives expression of mScarlet-I

Depositor

Richard Murray

Publication

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23101 and PphoA

Available since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap- TagIN-HA

Plasmid

#196491

Purpose

To tag endogenous mouse GFAP with HA

Depositor

Cagla Eroglu

Publication

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Inserts

HA
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptol

Plasmid

#218369

Purpose

Empty backbone for Tn7-site insertion with XylS-Pm expression system

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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