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pShuttle-CMV-Flag-SREBP-1c

Plasmid

#237348

Purpose

Adenoviral-SREBP-1c cleavage-activation reporter system

Depositor

Gerald Höfler

Article

Pena de la Sancha et al Commun Biol. 2025 Apr 9;8(1):588. doi: 1

Insert

SREBF1 (SREBF1 Human)

Use

Adenoviral and Mouse Targeting

Tags

Flag tag

Promoter

CMV

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-dCas9(sa)-VP64-AAV

Plasmid

#213035

Purpose

dCas9-VP64 expressed under control of TRE

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

dCas9

Use

AAV and CRISPR

Tags

NLS and NLS-VP64

Promoter

TRE, miniCMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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Cd99l2 long

Plasmid

#74469

Purpose

Expresses Cd99l2 transcript variant 1-IRES-EGFP under CAG promoter in mammalian cells

Depositor

Young Ho Suh

Article

Kang et al Cell Rep. 2025 Jan 28;44(1):115155. doi:

Insert

CD99 antigen-like 2, transcript variant 1 (Cd99l2 Mouse)

Expression

Mammalian

Promoter

chicken beta actin promoter

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only
Cd99l2 short

Plasmid

#74470

Purpose

Expresses Cd99l2 transcript variant 2-IRES-EGFP under CAG promoter in mammalian cells

Depositor

Young Ho Suh

Article

Kang et al Cell Rep. 2025 Jan 28;44(1):115155. doi:

Insert

CD99 antigen-like 2, transcript variant 2 (Cd99l2 Mouse)

Expression

Mammalian

Promoter

chicken beta actin promoter

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only
pDMJ2-SARS-CoV-2-Spike (B.1-9c)

Plasmid

#241053

Purpose

Expresses SARS-CoV-2 spike (B.1-9c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,R158A,T478K,E484P,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9a)

Plasmid

#241051

Purpose

Expresses SARS-CoV-2 spike (B.1-9a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F490R,S494R,D6…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9b)

Plasmid

#241052

Purpose

Expresses SARS-CoV-2 spike (B.1-9b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,T478K,E484V,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8c)

Plasmid

#241049

Purpose

Expresses SARS-CoV-2 spike (B.1-8c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8d)

Plasmid

#241050

Purpose

Expresses SARS-CoV-2 spike (B.1-8d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F490R,S494R,D6…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8a)

Plasmid

#241047

Purpose

Expresses SARS-CoV-2 spike (B.1-8a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,R158A,K147N,N439R,T478K,S494R,F490R,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8b)

Plasmid

#241048

Purpose

Expresses SARS-CoV-2 spike (B.1-8b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S155R,R403T,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-5a)

Plasmid

#241046

Purpose

Expresses SARS-CoV-2 spike (B.1-5a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-5a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,N481T,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-ITGB1(no stop)

Plasmid

#215453

Purpose

Gateway entry vector with integrin beta1 wild-type (WT) without a stop codon

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

Sept. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-BRAF-V600E-IRES-mCherry

Plasmid

#221026

Purpose

Fluorescent reporter for expressing a segment of BRAF-V600E CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

BRAF V600E (BRAF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pUltra BCR-ABL WT

Plasmid

#210432

Purpose

Lentiviral expression of BCR-ABL WT

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

BCR-ABL (ABL1, BCR Human)

Use

Lentiviral

Tags

EGFP-P2A

Promoter

UbC

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA893 - pBA904 Puro-T2A-BFP KLF5 g1 CRISPRa guide guide (pRCA594 backbone) 666

Plasmid

#238186

Purpose

Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 sgRNA CRISPRa (KLF5 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC4 (pP18(T)_C11-AVA4065)

Plasmid

#239302

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC4

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC4 (EXOSC4 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA860 - pBA904 Puro-T2A-GFP EGR3 g1 CRISPRa guide guide (pRCA360 backbone)

Plasmid

#238188

Purpose

Lentiviral CRISPR guide vector expressing a EGR3 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

EGR3 sgRNA CRISPRa (EGR3 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgNT-1-EF1a-LibVec

Plasmid

#239592

Purpose

expresses non-targeting (NT) guide#1

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

NT (non-targeting guide)

Use

AAV; To deliver a non-targeting guide (serving as…

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

Plasmid

#240228

Purpose

Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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